Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, USA.
Ann N Y Acad Sci. 2010 Jun;1197:178-83. doi: 10.1111/j.1749-6632.2009.05393.x.
Although the underlying molecular causes of aging are not entirely clear, hormetic agents like exercise, heat, and calorie restriction may generate a mild pro-oxidant stress that induces cell protective responses to promote healthy aging. As an individual ages, many cellular and physiological processes decline, including wound healing and reparative angiogenesis. This is particularly critical in patients with chronic non-healing wounds who tend to be older. We are interested in the potential beneficial effects of hyperbaric oxygen as a mild hormetic stress on human microvascular endothelial cells. We analyzed global gene expression changes in human endothelial cells following a hyperbaric exposure comparable to a clinical treatment. Our analysis revealed an upregulation of antioxidant, cytoprotective, and immediate early genes. This increase coincided with an increased resistance to a lethal oxidative stress. Our data indicate that hyperbaric oxygen can induce protection against oxidative insults in endothelial cells and may provide an easily administered hormetic treatment to help promote healthy aging.
虽然衰老的潜在分子原因尚不完全清楚，但应激剂，如运动、热和热量限制，可能会产生轻微的促氧化剂应激，诱导细胞保护性反应，促进健康衰老。随着个体衰老，许多细胞和生理过程会下降，包括伤口愈合和修复性血管生成。这在慢性非愈合性伤口的患者中尤为关键，因为他们往往年龄较大。我们对高压氧作为一种温和的应激剂对人微血管内皮细胞的潜在有益作用感兴趣。我们分析了人内皮细胞在高压暴露后的全基因表达变化，这种暴露与临床治疗相当。我们的分析显示，抗氧化剂、细胞保护和即刻早期基因上调。这种增加与对致命氧化应激的抵抗力增加同时发生。我们的数据表明，高压氧可以诱导内皮细胞对抗氧化损伤的保护作用，并且可以提供一种易于管理的应激治疗方法，以帮助促进健康衰老。
