Kleine Britta, Gatermann Sören, Sakinc Türkan
Institut für Hygiene und Mikrobiologie, Abteilung für Medizinische Mikrobiologie, Ruhr-Universität Bochum, D-44780 Bochum, Germany.
BMC Res Notes. 2010 Jun 10;3:163. doi: 10.1186/1756-0500-3-163.
The main aim of this study was to examine the genotypic and phenotypic diversity of Staphylococcus saprophyticus isolates from human and animal origin.
In total, 236 clinical isolates and 15 animal isolates of S. saprophyticus were characterized in respect of the occurrence of 9 potential virulence genes and four surface properties. All strains were PCR positive for the regulatory genes agr, sar >it>A and rot as well as for the surface proteins UafA and Aas. Nearly 90% of the clinical isolates were found to possess the gene for the surface-associated lipase Ssp and 10% for the collagen binding MSCRAMM SdrI. All animal isolates were negative forsdrI. Lipolytic activity could be detected in 66% of the clinical and 46% of the animal isolates. Adherence to collagen type I was shown of 20% of the clinical strains and 6% of the strains of animal origin. Most S. saprophyticus strains showed hydrophobic properties and only few could agglutinate sheep erythrocytes.
We described a broad analysis of animal and human S. saprophyticus isolates regarding virulence genes and phenotypic properties such as lipase activity, hydrophobicity, and adherence. While S. saprophyticus strains from animal sources have prerequisites for colonization of the urinary tract like the D-serine-deaminase, out findings suggested that they need to acquire new genes e.g. MSCRAMMS for adherence like sdrI and to modulate their existing properties e.g. increasing the lipase activity or reducing hydrophobicity. These apparently important new genes or properties for virulence have to be further analyzed.
本研究的主要目的是检测源自人和动物的腐生葡萄球菌分离株的基因型和表型多样性。
总共对236株临床分离株和15株动物源腐生葡萄球菌分离株进行了9种潜在毒力基因的出现情况及四种表面特性的鉴定。所有菌株的调控基因agr、sarA和rot以及表面蛋白UafA和Aas的PCR检测均呈阳性。近90%的临床分离株被发现拥有表面相关脂肪酶Ssp的基因，10%拥有胶原结合MSCRAMM SdrI的基因。所有动物分离株的sdrI基因均为阴性。66%的临床分离株和46%的动物分离株可检测到脂解活性。20%的临床菌株和6%的动物源菌株表现出对I型胶原的黏附性。大多数腐生葡萄球菌菌株表现出疏水性，只有少数能够凝集绵羊红细胞。
我们描述了对动物和人类腐生葡萄球菌分离株在毒力基因和表型特性如脂肪酶活性、疏水性和黏附性方面的广泛分析。虽然动物源腐生葡萄球菌菌株具备如D-丝氨酸脱氨酶等在泌尿道定植的先决条件，但我们的研究结果表明它们需要获得新的基因如用于黏附的MSCRAMM（如sdrI）并调节其现有特性，如增加脂肪酶活性或降低疏水性。这些对毒力显然重要的新基因或特性必须进一步分析。
