Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
BMC Res Notes. 2023 Sep 11;16(1):206. doi: 10.1186/s13104-023-06481-1.
The purpose of the present study was to investigate the biofilm production, and the presence of virulence genes and biochemical characteristics among the S. saprophyticus clinical isolates. A total of 35 clinical isolates of S. saprophyticus were collected from patients referred to several hospitals. By the crystal violet staining method, the capability of biofilm formation was performed. The genes associated with surface of S. saprophyticus were investigated by the PCR-sequencing techniques. Hemagglutination and lipase activity assays were also performed.
The results of crystal violet staining assay showed that 32 isolates (91%) form biofilm. Moreover, seven (20%), 13 (37%), and 12(34%) isolates were categorized as weak, moderate, and strong biofilm producers, respectively. virulence genes including UafA, Aas and Ssp had an overall prevalence of 88%, 91% and 80%, respectively. None of the isolates exhibited lipolytic activities. Regarding hemagglutination properties, only 11 (31%) isolates demonstrated hemagglutination of sheep erythrocytes. The results of this study indicate a high prevalence of UafA and Aas genes that can enhance the pathogenicity of S. saprophyticus, and Identification and better understanding of the functions of these genes can be used for therapeutic purposes. Maybe in the future we will be switch to anti-adhesion therapy because of drug resistance.
本研究旨在调查尿生殖支原体临床分离株的生物膜形成能力，以及毒力基因的存在和生化特性。从多家医院转来的患者中收集了 35 株尿生殖支原体临床分离株。采用结晶紫染色法检测生物膜形成能力。通过 PCR 测序技术研究与尿生殖支原体表面相关的基因。还进行了血凝和脂肪酶活性测定。
结晶紫染色试验结果显示，32 株（91%）形成生物膜。此外，7 株（20%）、13 株（37%）和 12 株（34%）分别为弱、中、强生物膜生产者。UafA、Aas 和 Ssp 等毒力基因的总流行率分别为 88%、91%和 80%。没有分离株显示脂肪酶活性。关于血凝特性，只有 11 株（31%）分离株对绵羊红细胞具有血凝性。本研究结果表明，UafA 和 Aas 基因的流行率很高，可增强尿生殖支原体的致病性。鉴定和更好地了解这些基因的功能可用于治疗目的。也许将来我们会因为耐药性而转向抗黏附治疗。
