Unité MycSA, UPR 1264, INRA, 71 Avenue Edouard Boeaux, BP 81, 33883 Villenave d'Ornon Cedex, France.
J Food Prot. 2010 Jun;73(6):1129-37. doi: 10.4315/0362-028x-73.6.1129.
The present study was aimed at characterizing the ability of lactic acid bacteria isolated from maize to repress the growth of fumonisin-producing fungi. A total of 67 isolates were screened for their antifungal activity against Fusarium proliferatum and Fusarium verticillioides by using the overlay method. The most efficient antifungal isolate was identified as Pediococcus pentosaceus (L006), on the basis of physiological and biochemical characterization and 16S rRNA gene sequencing. Production of the antifungal metabolite by this isolate commenced at the end of the growth exponential phase (8 h) and reached a maximum level after a long period of incubation (120 h). The antifungal metabolites produced were shown to be heat stable, resistant to proteolytic enzyme treatments, and pH dependent. The exact chemical nature of these substances remains to be clarified.
本研究旨在描述从玉米中分离出的乳酸菌抑制产伏马菌素真菌生长的能力。采用覆盖法，对 67 株分离株进行了抗真菌活性筛选，以对抗串珠镰刀菌和尖孢镰刀菌。根据生理生化特性和 16S rRNA 基因测序，最有效的抗真菌分离株被鉴定为戊糖片球菌（L006）。该分离株的抗真菌代谢产物的产生始于生长指数期结束时（8 h），并在长时间培养（120 h）后达到最高水平。所产生的抗真菌代谢产物具有热稳定性，能抵抗蛋白水解酶处理，并依赖 pH 值。这些物质的确切化学性质仍有待澄清。
