Nguyen Van Thi, Kwon Yong Min, Park Ae Ran, Yu Nan Hee, Choi Grace, Kim Jin-Cheol
Department of Agricultural Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Department of Biological Application and Technology, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea.
Mar Drugs. 2024 Nov 28;22(12):534. doi: 10.3390/md22120534.
This study explores the biocontrol potential of sp. M21F004, a lactic acid bacteria (LAB) isolated from marine environments, against several bacterial and fungal phytopathogens. Out of 50 marine bacterial isolates, sp. M21F004 was selected for its exceptional antimicrobial activity. The strain, isolated from the intestine of a starry flounder, was identified as sp. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that oleic acid (OA) is a key antimicrobial compound produced by sp. M21F004. In vitro assays showed that the culture broth (CB) of sp. M21F004, as well as OA, exhibited significant inhibitory effects against pathogens such as , , and subsp. . In vivo tests on cucumber wilt, creeping bentgrass dollar spot, tomato bacterial wilt, and kimchi cabbage soft rot further demonstrated the strain's efficacy in reducing disease severity. Moreover, OA had the highest control value of 74% against tomato bacterial wilt, followed by 64.1% against cucumber fusarium wilt, 42.5% against kimchi cabbage soft rot, and 16.5% against creeping bentgrass dollar spot. These findings suggest that sp. M21F004 and its metabolite OA offer promising alternatives to chemical pesticides, contributing to sustainable plant disease management by promoting resistance induction and providing an eco-friendly approach to agriculture.
本研究探讨了从海洋环境中分离出的乳酸杆菌（LAB）sp. M21F004对几种细菌和真菌植物病原体的生物防治潜力。在50株海洋细菌分离物中，sp. M21F004因其卓越的抗菌活性而被选中。该菌株从星斑川鲽的肠道中分离出来，被鉴定为sp.。气相色谱 - 质谱（GC - MS）分析表明，油酸（OA）是sp. M21F004产生的关键抗菌化合物。体外试验表明，sp. M21F004的培养液（CB）以及OA对诸如、、和亚种等病原体表现出显著的抑制作用。对黄瓜枯萎病、匍匐翦股颖币斑病、番茄青枯病和泡菜软腐病的体内试验进一步证明了该菌株在降低病害严重程度方面的功效。此外，OA对番茄青枯病的防治效果最高，为74%，其次是对黄瓜枯萎病的64.1%、对泡菜软腐病的42.5%和对匍匐翦股颖币斑病的16.5%。这些发现表明，sp. M21F004及其代谢产物OA为化学农药提供了有前景的替代品，通过促进抗性诱导和提供生态友好型农业方法，有助于实现可持续的植物病害管理。
