通过三维胶原基质中的基质硬度和孔隙率对细胞迁移活性的差异调节。
The differential regulation of cell motile activity through matrix stiffness and porosity in three dimensional collagen matrices.
机构信息
Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd Dallas, TX 75390-9039, USA.
出版信息
Biomaterials. 2010 Sep;31(25):6425-35. doi: 10.1016/j.biomaterials.2010.04.064.
In three dimensional collagen matrices, cell motile activity results in collagen translocation, cell spreading and cell migration. Cells can penetrate into the matrix as well as spread and migrate along its surface. In the current studies, we quantitatively characterize collagen translocation, cell spreading and cell migration in relationship to collagen matrix stiffness and porosity. Collagen matrices prepared with 1-4 mg/ml collagen exhibited matrix stiffness (storage modulus measured by oscillating rheometry) increasing from 4 to 60 Pa and matrix porosity (measured by scanning electron microscopy) decreasing from 4 to 1 microm(2). Over this collagen concentration range, the consequences of cell motile activity changed markedly. As collagen concentration increased, cells no longer were able to cause translocation of collagen fibrils. Cell migration increased and cell spreading changed from dendritic to more flattened and polarized morphology depending on location of cells within or on the surface of the matrix. Collagen translocation appeared to depend primarily on matrix stiffness, whereas cell spreading and migration were less dependent on matrix stiffness and more dependent on collagen matrix porosity.
在三维胶原基质中，细胞的运动活性导致胶原移位、细胞铺展和细胞迁移。细胞可以穿透基质，也可以在其表面铺展和迁移。在目前的研究中，我们定量地描述了胶原迁移、细胞铺展和细胞迁移与胶原基质硬度和孔隙率的关系。用 1-4mg/ml 胶原制备的胶原基质的基质硬度（通过振荡流变仪测量的储能模量）从 4pa 增加到 60pa，基质孔隙率（通过扫描电子显微镜测量）从 4μm(2)减少到 1μm(2)。在这个胶原浓度范围内，细胞运动活性的结果发生了显著变化。随着胶原浓度的增加，细胞不再能够引起胶原纤维的移位。细胞迁移增加，细胞铺展从树突状变为更平坦和极化的形态，这取决于细胞在基质内部或表面的位置。胶原迁移似乎主要取决于基质硬度，而细胞铺展和迁移对基质硬度的依赖性较小，对胶原基质孔隙率的依赖性较大。
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