Department of Biomedical Science, College of Life Science, CHA University 606-16, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
Biomaterials. 2010 Aug;31(24):6239-48. doi: 10.1016/j.biomaterials.2010.05.002.
Micro-structured scaffolds formed with poly(lactic- co -glycolic acid) (PLGA) microspheres were composed of adhesion molecules and growth factors. PLGA microspheres, constructed with Arg-Gly-Asp (RGD) peptide and bone morphogenic protein 2 (BMP-2) were created as a stem cell delivery vehicle. In this study, a high potential for cell adhesion and differentiation of human mesenchymal stem cells (hMSCs) was achieved by constructing the scaffolds with different compositions of coating materials. Specific gene and protein detection by RT-PCR and western blot analysis of the embedded hMSCs revealed that a combination of RGD peptide and BMP-2 induced differentiation of bone cells. Histology and immunohistochemistry results confirmed that bone cell-differentiated transplanted hMSCs were present in the micro-structured scaffolds. The results of this study demonstrate that the regulation of stem cell differentiation by adhesion molecules and growth factors has the potential to enable formation of therapeutic vehicles for the delivery of stem cells that are easily fabricated, less expensive, and more easily controlled than currently available delivery systems. The micro-structure typed PLGA microspheres used in this study possessed unique properties of ideal scaffolds. The embedded hMSCs easily adhered onto the PLGA microspheres mediated by RGD peptide, proliferated well onto the scaffolds, and differentiated to perform the distinct functions of bone tissues.
采用聚乳酸-共-羟基乙酸（PLGA）微球形成的微结构支架由黏附分子和生长因子组成。构建了含有精氨酸-甘氨酸-天冬氨酸（RGD）肽和骨形态发生蛋白 2（BMP-2）的 PLGA 微球，作为干细胞输送载体。在这项研究中，通过构建不同组成的涂层材料的支架，实现了人骨髓间充质干细胞（hMSCs）高黏附性和高分化能力。通过 RT-PCR 和 Western blot 分析嵌入的 hMSCs 的特定基因和蛋白检测，发现 RGD 肽和 BMP-2 的组合诱导了骨细胞的分化。组织学和免疫组织化学结果证实，分化的骨细胞来源的 hMSCs 存在于微结构支架中。这项研究的结果表明，黏附分子和生长因子对干细胞分化的调控具有形成治疗性载体的潜力，用于输送干细胞，这些载体比目前可用的输送系统更容易制造、更便宜、更易于控制。本研究中使用的微结构型 PLGA 微球具有理想支架的独特特性。通过 RGD 肽介导，嵌入的 hMSCs 很容易黏附在 PLGA 微球上，在支架上很好地增殖，并分化为具有独特组织功能的骨组织。
