Department of Psychology and Neuroscience, University of Colorado, Boulder, CO 80309-0345, USA.
J Neuroimmunol. 2010 Sep 14;226(1-2):181-4. doi: 10.1016/j.jneuroim.2010.05.022. Epub 2010 May 26.
The present study tested whether aging sensitizes hippocampal microglia to a pro-inflammatory challenge ex vivo. Hippocampal microglia from 3 and 24 mo old male F344 x BN F1 rats were exposed to LPS (0, 0.1, 1, 10 and 100 ng/ml) ex vivo. 2 h post-LPS challenge, gene expression of microglial activation markers and cytokines were assessed. 24 mo old animals exhibited a potentiated pro-inflammatory cytokine (IL-1β and IL-6) response to LPS and increased levels of CD11b, Iba-1 and MHCII irrespective of LPS treatment. The present results demonstrate that aging sensitizes hippocampal microglia to pro-inflammatory challenges.
本研究旨在测试衰老是否使海马小胶质细胞对体外的促炎刺激敏感。从 3 月龄和 24 月龄雄性 F344 x BN F1 大鼠的海马中分离小胶质细胞，进行 LPS（0、0.1、1、10 和 100ng/ml）体外刺激。LPS 刺激 2 小时后，评估小胶质细胞激活标志物和细胞因子的基因表达。24 月龄的动物对 LPS 表现出增强的促炎细胞因子（IL-1β 和 IL-6）反应，并且无论 LPS 处理与否，CD11b、Iba-1 和 MHCII 的水平均增加。本研究结果表明，衰老使海马小胶质细胞对促炎刺激敏感。
