Departamento de Veterinária, Universidade Federal de Viçosa, Campus s/n, Viçosa, MG 36570-000, Brazil.
Vet Parasitol. 2010 Aug 27;172(1-2):76-9. doi: 10.1016/j.vetpar.2010.04.034. Epub 2010 May 6.
The in vitro effect of four isolates of the nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34a) and Pochonia chlamydosporia (VC1 and VC4) on the eggs of Trichuris vulpis was evaluated. One thousand eggs of T. vulpis were plated on Petri dishes with 2% water-agar with the fungal isolates grown and without fungus as control. After 7, 14 and 21 days 100 eggs were removed from each plate and classified according to the following parameters: type 1, lytic effect without morphological damage to eggshell; type 2, lytic effect with morphological alteration of embryo and eggshell; and type 3, lytic effect with morphological alteration of embryo and eggshell, besides hyphal penetration and internal egg colonization. P. chlamydosporia demonstrated ovicidal activity (p<0.05) on the eggs of T. vulpis in the studied intervals presenting type 3 effect of 29.5% (VC1) and 36.5% (VC4), 59.5% (VC1) and 2.5% (VC4), 94.8% (VC1) and 2.95% (VC4) at 7, 14 and 21 days, respectively. The other fungi showed no type 3 effect. P. chlamydosporia should be a potential biological control agent of T. vulpis eggs.
评估了 4 株寄生线虫真菌——Duddingtonia flagrans (AC001)、Monacrosporium thaumasium (NF34a) 和 Pochonia chlamydosporia (VC1 和 VC4)——对犬复孔绦虫卵的体外效应。将 1000 个犬复孔绦虫卵接种在含有 2%水琼脂的培养皿中，培养皿中生长有真菌分离株和无真菌作为对照。在第 7、14 和 21 天，从每个平板中取出 100 个卵，并根据以下参数进行分类：1 型，无卵壳形态损伤的裂解作用；2 型，胚胎和卵壳形态改变的裂解作用；3 型，胚胎和卵壳形态改变的裂解作用，此外还有菌丝穿透和内部卵定植。在研究的间隔时间内，P. chlamydosporia 对犬复孔绦虫卵表现出杀卵活性（p<0.05），呈现 3 型效应，分别为 29.5%（VC1）和 36.5%（VC4）、59.5%（VC1）和 2.5%（VC4）、94.8%（VC1）和 2.95%（VC4），分别为第 7、14 和 21 天。其他真菌未表现出 3 型效应。P. chlamydosporia 可能是犬复孔绦虫卵的潜在生物防治剂。
