Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Vet Parasitol. 2010 Apr 19;169(1-2):123-7. doi: 10.1016/j.vetpar.2009.12.037. Epub 2010 Jan 4.
An assessment was made of the ovicidal activity of egg-parasitizing fungi Pochonia chlamydosporia (isolates VC1 and VC4) and Paecilomyces lilacinus on Toxocara canis eggs in vitro. The fungal isolates were inoculated onto Petri dishes with 2% water-agar (2% WA) and stored at 25 degrees C for 10 days in an incubator, in the dark. The control group was comprised of Petri dishes without fungi, containing the 2%WA medium only. Later, 1000 embryonated eggs were placed on the surface of the plates with fungal isolates and also on the control plates, and were then incubated at 25 degrees C for 7, 14 and 21 days. At these intervals, the eggs were retrieved and underwent percentage assessment according to the following parameters: no changes; type 1 effect, physiological and biochemical effect without morphological damage to eggshell, with visualization of hyphae adhered to eggshell; type 2 effect, lytic effect with morphological changes in embryo and eggshell, without hyphal penetration through the eggshell; type 3 effect, lytic effect with morphological changes in embryo and eggshell, with hyphal penetration and internal egg colonization. All the fungal isolates showed ovicidal activity (type 3 effect) on T. canis eggs, with 13.8%, 20.5% and 20.3% of ovicidal activity using P. chlamydosporia isolate VC1 after 7, 14 and 21 days, whereas isolate VC4 showed 15.2%, 19.0% and 21.7% of ovicidal activity at the same time intervals. P. lilacinus showed ovicidal activity of 12.3%, 18.8% and 20.0% after 7, 14 and 21 days. P. chlamydosporia and P. lilacinus were effective in vitro on T. canis eggs and can be considered a potential candidate to biological controller of those nematodes.
评估了寄生真菌多毛孢（分离株 VC1 和 VC4）和拟青霉对犬蛔虫卵的杀卵活性。将真菌分离物接种到含有 2%水琼脂（2%WA）的培养皿中，并在黑暗中于 25°C 的孵育箱中储存 10 天。对照组由不含真菌的培养皿组成，仅含有 2%WA 培养基。随后，将 1000 个胚胎卵放在含有真菌分离物的平板表面和对照平板上，然后在 25°C 下孵育 7、14 和 21 天。在这些时间间隔内，取回卵并根据以下参数进行百分比评估：无变化；1 型效应，无卵壳形态损伤的生理生化效应，可见附着在卵壳上的菌丝；2 型效应，卵壳形态发生裂解，胚胎和卵壳发生变化，菌丝未穿透卵壳；3 型效应，卵壳形态发生裂解，胚胎和卵壳发生变化，菌丝穿透并内部卵定植。所有真菌分离物对犬蛔虫卵均表现出杀卵活性（3 型效应），使用 P. chlamydosporia 分离株 VC1 后 7、14 和 21 天的杀卵活性分别为 13.8%、20.5%和 20.3%，而分离株 VC4 在相同时间间隔内的杀卵活性分别为 15.2%、19.0%和 21.7%。P. lilacinus 在 7、14 和 21 天后的杀卵活性分别为 12.3%、18.8%和 20.0%。P. chlamydosporia 和 P. lilacinus 对犬蛔虫卵具有体外杀卵活性，可作为这些线虫生物防治的潜在候选物。
