Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028, India.
Colloids Surf B Biointerfaces. 2010 Sep 1;79(2):521-3. doi: 10.1016/j.colsurfb.2010.04.022. Epub 2010 May 4.
The present work is a modification for Serpen et al.'s method for antioxidant activity determination for insoluble materials with special reference to metal oxide nanoparticles. As reported in our former publications the mixing procedures were followed as mentioned by Serpen et al. A new mixing procedure was followed for enhancing the reaction between metal oxide nanoparticles and DPPH in the solution. The sonication inside a water bath enhances the DPPH scavenging capacity of metal oxide nanoparticles up to 13.94% and 18.96% in case of ferric oxide and nickel oxide nanoparticles respectively. The control DPPH solution without any nanoparticles does not show any degradation due to sonication.
本工作是对 Serpen 等人用于测定不溶性物质抗氧化活性方法的改进，特别是针对金属氧化物纳米粒子。正如我们之前的出版物中所报道的，混合程序是按照 Serpen 等人所述进行的。为了增强金属氧化物纳米粒子与 DPPH 在溶液中的反应，我们采用了一种新的混合程序。在水浴中进行超声处理可分别将氧化铁和氧化镍纳米粒子对 DPPH 的清除能力提高 13.94%和 18.96%。没有任何纳米粒子的对照 DPPH 溶液由于超声处理不会显示出任何降解。
