Department of Immunology, University of Toronto and Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, Canada.
Semin Immunol. 2010 Oct;22(5):276-86. doi: 10.1016/j.smim.2010.04.014.
The thymus provides a unique set of cues for the effective selection and differentiation of CD4 and CD8 T-cells from a pool of immature thymocytes. Several approaches have been used to elucidate the complex set of intrathymic interactions that mediate these selection and differentiation events. Over the years, a number of different in vitro approaches have been developed and employed to assist in the identification of the cellular and molecular players that underpin thymic function. Here we provide an overview of the advances, which were obtained by in vitro approaches, to our understanding of the process of positive selection and CD4/CD8 lineage commitment.
胸腺为 CD4 和 CD8 T 细胞从未成熟的胸腺细胞中有效选择和分化提供了一组独特的线索。已经采用了几种方法来阐明介导这些选择和分化事件的复杂的胸腺内相互作用。多年来，已经开发和采用了许多不同的体外方法来协助确定支持胸腺功能的细胞和分子参与者。在这里，我们概述了通过体外方法获得的对阳性选择和 CD4/CD8 谱系决定过程的理解的进展。
