Department of Diagnostic Sciences, University of Texas Dental Branch at Houston, Houston, TX 77030, USA.
Microbes Infect. 2010 Oct;12(11):838-45. doi: 10.1016/j.micinf.2010.05.011. Epub 2010 Jun 9.
Porphyromonas gingivalis is etiologically associated with adult periodontitis, but it is unclear how P. gingivalis long-term interactions with bone cells contribute to this disease. This study investigates P. gingivalis interactions with osteoblasts over an extended time course. A primary mouse calvarial osteoblast culture was established and inoculated with P. gingivalis 33277 repeatedly every other day for up to four weeks. Invasion of osteoblasts by P. gingivalis, and the resulting effects on the proliferation, differentiation, and mineralization of osteoblasts were evaluated. P. gingivalis was found to invade osteoblasts in a dose-dependent manner, and repetitive inoculation increased the percentage of osteoblasts with internalized P. gingivalis. P. gingivalis did not affect osteoblast proliferation, but inhibited their differentiation and mineralization, partially via an inhibition of the differentiation regulatory transcription factors Cbfa-1 and osterix. In conclusion, P. gingivalis invades osteoblasts and inhibits bone formation, which likely contributes to alveolar bone loss in chronic periodontitis.
牙龈卟啉单胞菌与成人牙周炎有病因学关联，但尚不清楚牙龈卟啉单胞菌与骨细胞的长期相互作用如何导致这种疾病。本研究调查了牙龈卟啉单胞菌与成骨细胞的长期相互作用。建立了原代小鼠颅骨成骨细胞培养物，并每隔一天用牙龈卟啉单胞菌 33277 重复接种，持续长达四周。评估了牙龈卟啉单胞菌对成骨细胞的侵袭以及对成骨细胞增殖、分化和矿化的影响。结果发现，牙龈卟啉单胞菌以剂量依赖性方式侵袭成骨细胞，重复接种增加了内化牙龈卟啉单胞菌的成骨细胞百分比。牙龈卟啉单胞菌不影响成骨细胞增殖，但抑制其分化和矿化，部分通过抑制分化调节转录因子 Cbfa-1 和 osterix。总之，牙龈卟啉单胞菌侵袭成骨细胞并抑制骨形成，这可能导致慢性牙周炎中牙槽骨丧失。
