Kimura T, Ishikawa N, Shinkai K
Laboratory Animal Science, Sankyo Co., Ltd.
Arerugi. 1991 Mar;40(3 Pt 1):248-55.
It appears to be significant for the analytical studies of drug allergy that a comparison is made among inbred strains of mice, with different H-2 haplotypes with respect to the antibody response to low-molecular compounds. Such studies have been demonstrated by several investigators using high-molecular antigens, such as the synthetic polypeptides and natural proteins. The present experiments were undertaken, therefore, to clarify the differences in antibody responses to a low-molecular compound, TNBS in inbred strains of mice. Mice of four different strains, A/J (H-2a), C57BL/6 (H-2b), DBA/2 (H-2d) and C3H/He (H-2k) were immunized with TNBS (30 & 300 micrograms/body) plus FCA by the routes of ip or sc. Responses of IgG and IgE antibody productions were estimated by HA in serum samples treated with 2-ME and rat PCA reaction in untreated serum samples, respectively. As the results, DBA/2 (H-2d) mice showed high responses on IgE antibody production whereas C3H/He (H-2k) mice on IgG antibody production. A/J (H-2a) mice displayed high responses both on IgG and IgE antibody productions. C57BL/6 (H-2b) mice denoted low to intermediate responses both on IgG and IgE antibody productions. The above phenomenon may be interpreted by the fact that H-2a haplotype of A/J mice has a crossover on K region-E alpha subregion of H-2k and S.D regions of H-2d.
对于药物过敏的分析研究而言，在具有不同H - 2单倍型的近交系小鼠之间，就对低分子化合物的抗体反应进行比较似乎具有重要意义。几位研究人员已经使用高分子抗原，如合成多肽和天然蛋白质进行了此类研究。因此，本实验旨在阐明近交系小鼠对低分子化合物三硝基苯磺酸（TNBS）的抗体反应差异。将四种不同品系的小鼠，即A/J（H - 2a）、C57BL/6（H - 2b）、DBA/2（H - 2d）和C3H/He（H - 2k），通过腹腔注射或皮下注射途径用TNBS（30和300微克/只）加弗氏完全佐剂进行免疫。分别通过对用2 - 巯基乙醇处理的血清样本进行血凝试验（HA）和对未处理的血清样本进行大鼠被动皮肤过敏反应（PCA）来评估IgG和IgE抗体产生的反应。结果显示，DBA/2（H - 2d）小鼠在IgE抗体产生方面表现出高反应，而C3H/He（H - 2k）小鼠在IgG抗体产生方面表现出高反应。A/J（H - 2a）小鼠在IgG和IgE抗体产生方面均表现出高反应。C57BL/6（H - 2b）小鼠在IgG和IgE抗体产生方面均表现出低至中等反应。上述现象可能是由于A/J小鼠的H - 2a单倍型在H - 2k的K区 - Eα亚区和H - 2d的S.D区存在交叉。
