Neurosciences Program, Stanford University, Stanford, California 94305-4075, USA.
J Neurophysiol. 2010 Aug;104(2):799-810. doi: 10.1152/jn.00231.2009. Epub 2010 Jun 10.
Dorsal premotor cortex (PMd) is known to be involved in the planning and execution of reaching movements. However, it is not understood how PMd plan activity-often present in the very same neurons that respond during movement-is prevented from itself producing movement. We investigated whether inhibitory interneurons might "gate" output from PMd, by maintaining high levels of inhibition during planning and reducing inhibition during execution. Recently developed methods permit distinguishing interneurons from pyramidal neurons using extracellular recordings. We extend these methods here for use with chronically implanted multi-electrode arrays. We then applied these methods to single- and multi-electrode recordings in PMd of two monkeys performing delayed-reach tasks. Responses of putative interneurons were not generally in agreement with the hypothesis that they act to gate output from the area: in particular it was not the case that interneurons tended to reduce their firing rates around the time of movement. In fact, interneurons increased their rates more than putative pyramidal neurons during both the planning and movement epochs. The two classes of neurons also differed in a number of other ways, including greater modulation across conditions for interneurons, and interneurons more frequently exhibiting increases in firing rate during movement planning and execution. These findings provide novel information about the greater responsiveness of putative PMd interneurons in motor planning and execution and suggest that we may need to consider new possibilities for how planning activity is structured such that it does not itself produce movement.
背侧运动前皮层(PMd)已知参与了运动的规划和执行。然而,目前尚不清楚 PMd 如何规划活动——通常存在于对运动做出响应的相同神经元中——防止活动本身产生运动。我们研究了抑制性中间神经元是否可以通过在规划期间保持高水平的抑制和在执行期间降低抑制来“控制”PMd 的输出。最近开发的方法允许使用细胞外记录来区分中间神经元和锥体神经元。我们在此将这些方法扩展到使用慢性植入的多电极阵列。然后,我们将这些方法应用于两只猴子在执行延迟到达任务时在 PMd 中的单电极和多电极记录。假定中间神经元的反应通常与它们充当区域输出门的假设不一致:特别是,中间神经元并没有倾向于在运动时降低其放电率。事实上,在规划和运动时期,中间神经元的放电率比假定的锥体神经元增加得更多。这两类神经元在许多其他方面也有所不同,包括中间神经元的条件调制更大,以及中间神经元在运动规划和执行期间更频繁地增加放电率。这些发现提供了关于在运动规划和执行中假定 PMd 中间神经元响应性更高的新信息,并表明我们可能需要考虑规划活动的结构的新可能性,以便它本身不会产生运动。