Centre for Biological Safety, Robert Koch Institute, Berlin, Germany.
Melanoma Res. 2010 Oct;20(5):435-40. doi: 10.1097/CMR.0b013e32833c1b5d.
Human endogenous retroviruses (HERVs) are discussed as causative agents of various diseases including cancers. Expression of endogenous retroviral sequences can be induced by ultraviolet (UV) light, which is also considered as a cofactor in the development of cutaneous melanoma. Therefore, we investigated whether ultraviolet C (UVC) induces HERV-K rec and np9 expression in normal human epidermal melanocytes (NHEM) and in melanoma cell lines. NHEM and four different melanoma cell lines were irradiated with 10 and 30 mJ/cm(2) UVC, respectively. Expression of the HERV-K transcripts rec and np9 was measured 0, 6, 12, and 24 h after UV exposure by quantitative real-time PCR. In NHEM, HERV-K rec expression was significantly induced 24 h after UV exposure with 10 mJ/cm(2) UVC, whereas np9 expression transiently increased 6 and 12 h after irradiation with both UV doses. In contrast, in melanoma cell lines MEWO, G-361 and GR-M rec, and np9 expression was downregulated or remained unchanged after UV treatment. UVC irradiation induced HERV-K rec and np9 expression in NHEM, which might be an indicator of a functional role of Rec and/or Np9 in melanoma formation.
人类内源性逆转录病毒（HERV）被认为是多种疾病（包括癌症）的致病因子。内源性逆转录病毒序列的表达可以被紫外线（UV）诱导，紫外线也被认为是皮肤黑色素瘤发展的一个协同因素。因此，我们研究了紫外线 C（UVC）是否会诱导正常人类表皮黑素细胞（NHEM）和黑色素瘤细胞系中 HERV-K rec 和 np9 的表达。用 10 和 30 mJ/cm2 的 UVC 分别辐照 NHEM 和四种不同的黑色素瘤细胞系。用定量实时 PCR 测量 UV 暴露后 0、6、12 和 24 小时 HERV-K 转录物 rec 和 np9 的表达。在 NHEM 中，用 10 mJ/cm2 的 UVC 照射 24 小时后，HERV-K rec 的表达明显被诱导，而 np9 的表达在两种剂量的 UV 照射后 6 和 12 小时短暂增加。相比之下，在黑色素瘤细胞系 MEWO、G-361 和 GR-M 中，rec 和 np9 的表达在 UV 处理后下调或保持不变。UVC 照射诱导 NHEM 中 HERV-K rec 和 np9 的表达，这可能是 Rec 和/或 Np9 在黑色素瘤形成中具有功能作用的一个指标。
