Tonoue T, Kitoh J
Endocrinol Jpn. 1978 Feb;25(1):105-9. doi: 10.1507/endocrj1954.25.105.
The thyroid lobes excised from one-day-old or 13-day-old chickens were incubated with or without TSH, and cAMP in the medium was determined. TSH induced the release of cAMP from thyroid lobes, resulting in the increase of cAMP concentration in the incubating medium. The release of cAMP into the incubating media in the presence of TSH from the thyroids of the chickens which were pretreated with TSH neonatally was more marked as compared to that from the thyroids of the control chickens. The light and electron microscopic examination of the thyroid lobes incubated with TSH showed that the morphological changes such as colloid droplets formation and enlargement of endoplasmic reticulum were induced.
将1日龄或13日龄鸡的甲状腺叶进行孵育，孵育时添加或不添加促甲状腺激素（TSH），并测定培养基中的环磷酸腺苷（cAMP）含量。TSH可诱导甲状腺叶释放cAMP，导致孵育培养基中cAMP浓度升高。与对照鸡的甲状腺相比，新生期经TSH预处理的鸡的甲状腺在TSH存在下向孵育培养基中释放cAMP的情况更为明显。对经TSH孵育的甲状腺叶进行光镜和电镜检查发现，可诱导出诸如胶体小滴形成和内质网扩张等形态学变化。
