Chen Qian, Shi Sheng-Yun, Hu Zheng, Zhang Qi-Hua, Cao Xin
Institute of Pediatrics, Nanjing Children's Hospital, Nanjing Medical University, Nanjing 210008, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2010 Jun;12(6):450-4.
To investigate the nonbacterial pathogens in children with acute respiratory infection (ARI) in Nanjing.
The presence of Mycoplasma pneumoniae (MP) and Chlamydia trachomatis (CT) was determined by quantitative PCR in the nasopharyngeal samples from 1 592 hospitalized children with ARI. Common respiratory viruses, including respiratory syncytial virus (RSV), adenovirus (ADV), influenza virus types A and B (IVA and IVB), parainfluenza virus types 1, 2, 3(PIV-1, 2, 3) and human metapneumovirus (hMPV), were detected using direct immunofluorescence assay.
MP and CT were detected in 25.7% and 2.4% of the 1 592 samples respectively. The overall positive rate of respiratory viruses was 40.9%. Among the viruses, the top detected virus was RSV (61.3%), followed by PIV-3 (6.7%) and hMPV (4.9%). Mixed infection among MP, CT and viruses was observed in 107 cases (6.7%). The infants under 1 year old were susceptible to mix-infection (68/107, 63.6%).
Respiratory virus is the main pathogen responsible for ARI in children from Nanjing. RSV is the most commonly identified virus. MP is also the frequently identified pathogen for ARI in children. Mixed infection is common in infants under 1 year old.
调查南京地区急性呼吸道感染（ARI）患儿中的非细菌性病原体。
采用定量聚合酶链反应（PCR）检测1592例住院ARI患儿鼻咽部样本中肺炎支原体（MP）和沙眼衣原体（CT）的存在情况。采用直接免疫荧光法检测常见呼吸道病毒，包括呼吸道合胞病毒（RSV）、腺病毒（ADV）、甲型和乙型流感病毒（IVA和IVB）、1、2、3型副流感病毒（PIV-1、2、3）和人偏肺病毒（hMPV）。
1592份样本中MP和CT的检出率分别为25.7%和2.4%。呼吸道病毒总阳性率为40.9%。在这些病毒中，检出率最高的是RSV（61.3%），其次是PIV-3（6.7%）和hMPV（4.9%）。共观察到107例（6.7%）MP、CT与病毒混合感染。1岁以下婴儿易发生混合感染（68/107，63.6%）。
呼吸道病毒是南京地区儿童ARI的主要病原体。RSV是最常见的病毒。MP也是儿童ARI常见的病原体。1岁以下婴儿混合感染常见。
