Graduate School of Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan.
J Biosci Bioeng. 2010 Jul;110(1):113-7. doi: 10.1016/j.jbiosc.2010.01.007. Epub 2010 Jan 29.
The effects of fucose-containing sulfated polysaccharides, ascophyllan and fucoidan, isolated from the brown alga Ascophyllum nodosum, on the growth of various cell lines (MDCK, Vero, PtK(1), CHO, HeLa, and XC) were investigated. In a colony formation assay, ascophyllan and fucoidan showed potent cytotoxic effects on Vero and XC cells, while other cell lines were relatively resistant to these polysaccharides. Almost no significant effects of these polysaccharides were observed in the cell lines tested using the Alamar blue cytotoxicity assay over 48 h with varying initial cell densities (2500-20,000 cells/well) in growth medium. Interestingly, a significant growth promoting effect of ascophyllan on MDCK cells was observed, whereas treatment with fucoidan showed growth suppressive effects on this cell line under the same experimental conditions. These results suggest that ascophyllan is distinguishable from fucoidan in terms of their bioactivities. This is the first report of the growth promoting effects of a sulfated fucan on a mammalian cell line under normal growth conditions.
从褐藻马尾藻中分离得到的含岩藻糖的硫酸多糖，藻朊酸钠和褐藻糖胶，对各种细胞系（MDCK、Vero、PtK(1)、CHO、HeLa 和 XC）的生长的影响进行了研究。在集落形成试验中，藻朊酸钠和褐藻糖胶对 Vero 和 XC 细胞表现出强烈的细胞毒性作用，而其他细胞系对这些多糖相对具有抗性。在含有不同初始细胞密度（2500-20,000 个细胞/孔）的生长培养基中，用 Alamar blue 细胞毒性测定法在 48 小时内检测到，这些多糖在测试的细胞系中几乎没有明显的作用。有趣的是，在相同的实验条件下，藻朊酸钠对 MDCK 细胞有明显的促进生长作用，而褐藻糖胶处理则对该细胞系有生长抑制作用。这些结果表明，藻朊酸钠和褐藻糖胶在生物活性方面是有区别的。这是在正常生长条件下，关于硫酸化岩藻聚糖对哺乳动物细胞系的生长促进作用的首次报道。
Zotero 插件