Nakayasu Seiichirou, Soegima Ryo, Yamaguchi Kenichi, Oda Tatsuya
Research and Development Division, Hayashikane Sangyo Co., Ltd., Shimonoseki, Yamaguti 750-8608, Japan.
Biosci Biotechnol Biochem. 2009 Apr 23;73(4):961-4. doi: 10.1271/bbb.80845. Epub 2009 Apr 7.
A fucose-containing, sulfated polysaccharide ascophyllan was isolated from the brown alga Ascophyllum nodosum. Composition analysis demonstrated that ascophyllan mainly contains uronic acid, xylose, fucose, and sulfate half ester in approximately equimolecular proportions, which are evidently distinct from those of alginate and fucoidan. Ascophyllan inhibited the proliferation of U937 cells in a concentration-dependent manner, and DNA-fragmentation and typical apoptotic nuclear morphological changes were observed in the ascophyllan-treated cells. Furthermore, ascophyllan induced the secretion of tumor necrosis factor-alpha (TNF-alpha) and granulocyte colony-stimulating factor (G-CSF) from mouse macrophage cell line RAW264.7.
从褐藻泡叶藻中分离出一种含岩藻糖的硫酸化多糖——泡叶藻多糖。成分分析表明，泡叶藻多糖主要含有糖醛酸、木糖、岩藻糖和硫酸半酯，其分子比例大致相等，这明显不同于藻酸盐和岩藻依聚糖。泡叶藻多糖以浓度依赖的方式抑制U937细胞的增殖，并且在经泡叶藻多糖处理的细胞中观察到DNA片段化和典型的凋亡核形态变化。此外，泡叶藻多糖诱导小鼠巨噬细胞系RAW264.7分泌肿瘤坏死因子-α（TNF-α）和粒细胞集落刺激因子（G-CSF）。
