Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, Nagasaki, Japan.
Research and Development Division, Hayashikane Sangyo Co., Ltd., Yamaguchi, Japan.
Biosci Biotechnol Biochem. 2021 Mar 24;85(4):882-889. doi: 10.1093/bbb/zbaa115.
In this study, we found that a sulfated polysaccharide isolated from the brown alga Ascophyllum nodosum, ascophyllan, showed suppressive effects on stimulated RAW264.7 cells. Ascophyllan significantly inhibited expression of inducible nitric oxide synthase mRNA and excessive production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells in a dose-dependent manner without affecting the viability of RAW264.7 cells. Ascophyllan also reduced the elevated level of intracellular reactive oxygen species (ROS) in LPS-stimulated RAW264.7 cells. Furthermore, preincubation with ascophyllan resulted in concentration-dependent decrease in ROS production in phorbol 12-myristate-13-acetate-stimulated RAW264.7 cells. Our results suggest that ascophyllan can exhibit anti-inflammatory effects on stimulated macrophages mainly through the attenuation of NO and ROS productions.
在这项研究中，我们发现一种从褐藻裙带菜中分离得到的硫酸多糖——裙带菜聚糖，对受刺激的 RAW264.7 细胞具有抑制作用。裙带菜聚糖能显著抑制诱导型一氧化氮合酶 mRNA 的表达，抑制脂多糖（LPS）刺激的 RAW264.7 细胞过量产生一氧化氮（NO），且对 RAW264.7 细胞的活力无影响。裙带菜聚糖还能降低 LPS 刺激的 RAW264.7 细胞内活性氧（ROS）水平的升高。此外，用裙带菜聚糖预处理可使佛波醇 12-肉豆蔻酸 13-乙酸酯刺激的 RAW264.7 细胞中 ROS 的产生呈浓度依赖性降低。我们的研究结果表明，裙带菜聚糖对受刺激的巨噬细胞具有抗炎作用，主要是通过抑制 NO 和 ROS 的产生。
