Division of Biochemistry, Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Nagasaki 852-8521, Japan.
Int J Biol Macromol. 2013 Aug;59:305-12. doi: 10.1016/j.ijbiomac.2013.04.035. Epub 2013 May 2.
Antioxidant activities of sulfated polysaccharide ascophyllan from Ascophyllum nodosum was investigated in vitro by various assays, and compared with those of fucoidan. A chemiluminescence (CL) analysis using a luminol analog, L-012, showed that ascophyllan scavenges superoxide, and the activity is greater than fucoidan. However, in the presence of 10μg/ml of ascophyllan or 10μg/ml and 100μg/ml of fucoidan, slightly enhanced CL-responses were observed. Since EDTA-treatment resulted in disappearance of the enhancement effects, it was suggested that metal ions especially iron ions in the polysaccharides might be involved in this phenomenon. In fact, metal element analysis revealed that ascophyllan and fucoidan inherently contain iron and other metal elements. EDTA-treatment resulted in significant increase in Fe(2+)-chelating activities of these polysaccharides. In an electron spin resonance (ESR)-spin trapping analysis in which direct UV-radiation to hydrogen peroxide was used as a source of hydroxyl radical, ascophyllan and fucoidan showed potent hydroxyl radical scavenging activity with similar extent. Reducing power of ascophyllan was stronger than that of fucoidan. Our results indicate that ascophyllan can exhibit direct and potent antioxidant activity.
从裙带菜中提取的硫酸化多糖岩藻聚糖的抗氧化活性通过各种方法在体外进行了研究，并与褐藻糖胶的抗氧化活性进行了比较。使用发光氨酸类似物 L-012 进行的化学发光（CL）分析表明，岩藻聚糖能清除超氧阴离子，其活性大于褐藻糖胶。然而，在存在 10μg/ml 岩藻聚糖或 10μg/ml 和 100μg/ml 褐藻糖胶的情况下，观察到略微增强的 CL 反应。由于 EDTA 处理导致增强效应消失，因此表明多糖中的金属离子，特别是铁离子可能参与了这一现象。事实上，金属元素分析表明，岩藻聚糖和褐藻糖胶本身含有铁和其他金属元素。EDTA 处理导致这些多糖的 Fe(2+)-螯合活性显著增加。在电子自旋共振（ESR）-自旋捕获分析中，直接用紫外线辐射过氧化氢作为羟自由基的来源，岩藻聚糖和褐藻糖胶表现出相似程度的强大羟自由基清除活性。岩藻聚糖的还原能力强于褐藻糖胶。我们的结果表明，岩藻聚糖可以表现出直接而有效的抗氧化活性。
