GnRH II和GnRH I对子宫内膜异位症患者在位及异位子宫内膜间质细胞分泌血管内皮生长因子的影响。

Effect of GnRH II and GnRH I on secretion of VEGF by eutopic and ectopic endometrial stromal cells of endometriosis patients.

作者信息

Huang Fengying, Liu Qiuhong, Wang Huanping, Zou Ying

机构信息

Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, Changsha 410011, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2010 May;35(5):409-18. doi: 10.3969/j.issn.1672-7347.2010.05.002.

DOI:10.3969/j.issn.1672-7347.2010.05.002

PMID:20543462

Abstract

OBJECTIVE

To determine the effect of GnRH I and GnRH II on the secretion of VEGF by eutopic and ectopic endometrial stromal cells cultured in vitro, and to provide theoretical basis for exploring new treatments for endometriosis (EMs).

METHODS

Eutopic and ectopic endometrium stromal cells cultured in vitro were treated with different concentrations of GnRH II and a GnRH I (goserelin), and a control group was not treated by GnRH II and GnRH I. Enzyme linked immunosorbent assay (ELISA) was used to measure the content of vascular endothelial growth factor (VEGF) protein in the medium of the above 2 groups.

RESULTS

(1) There was no difference in the VEGF protein secreted by eutopic and ectopic stromal cells in the medium after being cultured in vitro for 48 h (P>0.05). (2) 10(-10), 10(-8), and 10(-6) mol/L GnRH II dose-dependently reduced VEGF protein secreted by endometrial stromal cells (P<0.05),and the inhibition effect was stronger than that of GnRH I (P<0.05).(3)The inhibition effect of GnRH II on VEGF in ectopic stromal cells was stronger than that of eutopic stromal cells (P<0.05).

CONCLUSION

(1) Ectopic stromal cells cultured in vitro can secrete VEGF,which has no difference from the eutopic stromal cells, and which may play an important role in the formation and development of EMs. (2) GnRH II can dose-dependently reduce VEGF protein secreted by ectopic and eutopic endometrial stromal cells cultured in vitro,and the inhibition effect is stronger than that of GnRH I, providing theoretical basis for exploring new treatments for EMs.

摘要

目的

探讨促性腺激素释放激素I（GnRH I）和促性腺激素释放激素II（GnRH II）对体外培养的在位及异位子宫内膜基质细胞分泌血管内皮生长因子（VEGF）的影响，为探索子宫内膜异位症（EMs）的新治疗方法提供理论依据。

方法

体外培养在位和异位子宫内膜基质细胞，分别用不同浓度的GnRH II和GnRH I（戈舍瑞林）处理，对照组不做处理。采用酶联免疫吸附测定法（ELISA）检测上述两组细胞培养液中VEGF蛋白含量。

结果

（1）体外培养48 h后，在位和异位基质细胞分泌的VEGF蛋白含量差异无统计学意义（P>0.05）。（2）10^(-10)、10^(-8)、10^(-6) mol/L GnRH II可使子宫内膜基质细胞分泌的VEGF蛋白含量呈剂量依赖性降低（P<0.05），且抑制作用强于GnRH I（P<0.05）。（3）GnRH II对异位基质细胞VEGF的抑制作用强于在位基质细胞（P<0.05）。