Li Xiao-yan, Leng Jin-hua, Lang Jing-he
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing 100730, China.
Zhonghua Fu Chan Ke Za Zhi. 2012 Jun;47(6):440-4.
To investigate the influence of gonadotropin releasing hormone agonist (GnRH-a) on the expression mRNA of nerve growth factor (NGF) and its receptors (TrkA and P75NTR) in normal and eutopic endometrial stromal cells (ESC).
From January to April 2009, 3 patients with endometriosis undergoing surgery in Peking Union Medical College Hospital were obtained eutopic endometrium as study group matched with eutopic endometrium from 3 patients with teratoma as control group. ESC were incubated with different concentration of GnRH-a (0, 5×10⁻¹¹, 5×10⁻¹⁰, 5×10⁻⁹, 5×10⁻⁸, 5×10⁻⁷ g/ml). The expression of mRNA of NGF, TrkA and P75NTR were measured by real-time-PCR.
At concentration of 0 g/ml, the levels of NGF, TrkA and P75NTR mRNA in ESC were 6.32, 8.55, 8.08 in study group, which were significantly higher than 0.94, 0.67, 1.08 in control group (P < 0.05). Treated by the following concentration of GnRH-a (5×10⁻¹¹, 5×10⁻¹⁰, 5×10⁻⁹, 5×10⁻⁸, 5×10⁻⁷ g/ml), the median expression of NGF, TrkA and P75NTR mRNA was 1.00, 0.96, 1.05; 1.09, 0.82, 1.27; 1.04, 0.52, 0.81;1.00, 0.55, 0.64; 0.78, 0.49, 1.02 in study group. Compared with the expressions of those untreated by GnRH-a in study group, they showed significantly lower trends (P < 0.05). In control group, the median expression of NGF, TrkA and P75NTR mRNA was 0.98, 0.37, 0.92; 0.70, 0.45, 1.15; 1.55, 0.80, 1.35; 1.09, 0.41, 1.35; 0.90, 0.82, 1.18. Compared with the expressions of those untreated by GnRH-a in control group, there were no statistically differences (P > 0.05). And treated by the same concentration of GnRH-a, the expressions of NGF, TrkA and P75NTR mRNA did not show statistically difference between the two groups (P > 0.05).
The expression of NGF, TrkA and P75NTR mRNA were suppressed by GnRH-a.
探讨促性腺激素释放激素激动剂（GnRH-a）对正常及在位子宫内膜间质细胞（ESC）中神经生长因子（NGF）及其受体（TrkA和P75NTR）mRNA表达的影响。
2009年1月至4月，选取北京协和医院3例因子宫内膜异位症行手术治疗患者的在位子宫内膜作为研究组，选取3例畸胎瘤患者的在位子宫内膜作为对照组。将ESC与不同浓度的GnRH-a（0、5×10⁻¹¹、5×10⁻¹⁰、5×10⁻⁹、5×10⁻⁸、5×10⁻⁷ g/ml）孵育。采用实时荧光定量PCR检测NGF、TrkA和P75NTR mRNA的表达。
在0 g/ml浓度时，研究组ESC中NGF、TrkA和P75NTR mRNA水平分别为6.32、8.55、8.08，显著高于对照组的0.94、0.67、1.08（P<0.05）。经以下浓度的GnRH-a（5×；10⁻¹¹、5×10⁻¹⁰、5×10⁻⁹、5×10⁻⁸、5×10⁻⁷ g/ml）处理后，研究组中NGF、TrkA和P75NTR mRNA的中位表达量分别为1.00、0.96、1.05；1.09、0.82、1.27；1.04、0.52、0.81；1.00、0.55、0.64；0.78、0.49、；1.02。与研究组中未用GnRH-a处理的表达量相比，呈显著降低趋势（P<0.05）。对照组中，NGF、TrkA和P75NTR mRNA的中位表达量分别为0.98、0.37、0.92；0.70、0.45、1.15；1.55、0.80、1.35；1.09、0.41、1.35；0.90、0.82、1.18。与对照组中未用GnRH-a处理的表达量相比，差异无统计学意义（P>0.05）。并且用相同浓度的GnRH-a处理后，两组间NGF、TrkA和P75NTR mRNA的表达无统计学差异（P>0.05）。
GnRH-a可抑制NGF、TrkA和P75NTR mRNA的表达。
