[促性腺激素释放激素II和促性腺激素释放激素Ia对子宫内膜异位症患者基质细胞增殖的抑制作用]
[Inhibitory effect of GnRHII and GnRH Ia on the stromal cell proliferation from endometriosis patients].
作者信息
Huang Fengying, Wang Huanping, Wu Mei, Yin Tuanfang
机构信息
Department of Obstetrics and Gyneology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Jun;36(6):554-8. doi: 10.3969/j.issn.1672-7347.2011.06.013.
OBJECTIVE
To investigate the inhibitory effect of gonadotropin-releasing hormone II(GnRHII) and gonadotropin-releasing hormone I agonist (GnRH Ia) on the proliferation of endometrial stromal cells in vitro from endometriosis patients.
METHODS
Different concentrations of GnRHII or GnRH Ia were added into the cultured endometrial stromal cells in vitro to detect the cell proliferation inhibition by MTT test.
RESULTS
The inhibitory rate of GnRHII or GnRH Ia on eutopic and ectopic endometrial stromal cells in vitro was both dose- and time-dependent (P<0.05). Effect of GnRHII or GnRH Ia on the inhibitory rate of ectopic endometrial stromal cells was significantly higher than that of eutopic (P<0.05). GnRH II had a higher inhibitory rate on the endometrial stromal cells in vitro than did GnRH Ia (P<0.05).
CONCLUSION
GnRHII has more antiproliferative effect on endometrial stromal cells than GnRH Ia in vitro, especially on ectopic endometrial stromal cells, suggesting that GnRHII may be more effective than GnRH Ia on endometriosis.
目的
探讨促性腺激素释放激素II（GnRHII）和促性腺激素释放激素I激动剂（GnRH Ia）对子宫内膜异位症患者体外子宫内膜基质细胞增殖的抑制作用。
方法
将不同浓度的GnRHII或GnRH Ia加入体外培养的子宫内膜基质细胞中，通过MTT试验检测细胞增殖抑制情况。
结果
GnRHII或GnRH Ia对体外在位和异位子宫内膜基质细胞的抑制率均呈剂量和时间依赖性（P<0.05）。GnRHII或GnRH Ia对异位子宫内膜基质细胞抑制率的影响显著高于在位细胞（P<0.05）。GnRH II对体外子宫内膜基质细胞的抑制率高于GnRH Ia（P<0.05）。
结论
体外实验中，GnRHII对子宫内膜基质细胞的抗增殖作用比GnRH Ia更强，尤其是对异位子宫内膜基质细胞，提示GnRHII对子宫内膜异位症的治疗可能比GnRH Ia更有效。
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