Institute of Endocrinology, Metabolism and Hypertension, Tel-Aviv Sourasky Medical Centre, 6 Weizmann street, Tel-Aviv 64239, Israel.
J Endocrinol Invest. 2011 Feb;34(2):106-10. doi: 10.1007/BF03347039. Epub 2010 Jun 11.
We demonstrated previously that phytoestrogens and vitamin D analogs like estradiol-17β (E2) modulate bone morphology in rat female model.
We now analyze the effects of phytoestrogens, E2, selective E2 re ceptor modulators, and the less-calcemic analogs of vitamin D: JKF1624F2-2 (JKF) or QW1624F2-2 (QW) on fat content in bone marrow (BM) from long bones in ovariectomized female rats (OVX).
OVX rats were injected with treatments known to affect bone formation, 5 days per week for 2.5 month for analysis of fat content in BM.
In OVX young adults there is a decreased bone formation and a 10-fold increase in fat cells content in BM. Treatment with E2, raloxifene (Ral) or DT56a resulted in almost completely abolishment of fat cells content. Daidzein (D) decreased fat cells content by 80%, genistein (G) or biochainin A (BA) did not change fat cells content and carboxy BA (cBA) had a small but significant effect. JKF or QW did not affect fat cells content, whereas combined treatment of JKF or QW with E2 resulted in complete abolishment of fat cells content. These changes in fat cells content are inversely correlated with changes in bone formation.
Our results demonstrate that adipogenesis induced by OVX is a reversible process which can be corrected by hormonal treatments. The awareness of a relationship between fat and bone at the marrow level might provide a better understanding of the pathophysiology of bone loss as well as a novel approach to diagnosis and treatment of postmenopausal osteoporosis.
我们之前已经证明，植物雌激素和维生素 D 类似物，如雌二醇-17β（E2），可以调节雌性大鼠模型的骨骼形态。
我们现在分析植物雌激素、E2、选择性 E2 受体调节剂以及维生素 D 的低钙类似物：JKF1624F2-2（JKF）或 QW1624F2-2（QW）对去卵巢雌性大鼠（OVX）长骨骨髓（BM）中脂肪含量的影响。
OVX 大鼠每周接受 5 次已知能影响骨形成的治疗，共 2.5 个月，用于分析 BM 中的脂肪含量。
在年轻的 OVX 成年大鼠中，骨形成减少，BM 中的脂肪细胞含量增加了 10 倍。用 E2、雷洛昔芬（Ral）或 DT56a 治疗可使脂肪细胞含量几乎完全消失。大豆苷元（D）使脂肪细胞含量减少 80%，染料木黄酮（G）或生物链素 A（BA）不改变脂肪细胞含量，而羧基 BA（cBA）则有较小但显著的作用。JKF 或 QW 不影响脂肪细胞含量，而 JKF 或 QW 与 E2 联合治疗可使脂肪细胞含量完全消失。脂肪细胞含量的这些变化与骨形成的变化呈负相关。
我们的结果表明，OVX 诱导的脂肪生成是一个可逆转的过程，可以通过激素治疗来纠正。骨髓水平脂肪和骨骼之间的关系的认识可能会更好地理解骨丢失的病理生理学，以及为绝经后骨质疏松症的诊断和治疗提供新的方法。
