Inoue Yasuhiro, Yokobori Takehiko, Yokoe Takeshi, Toiyama Yuji, Miki Chikao, Mimori Koshi, Mori Masaki, Kusunoki Masato
Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, Mie, Japan.
Ann Surg Oncol. 2010 Nov;17(11):3037-42. doi: 10.1245/s10434-010-1132-y. Epub 2010 Jun 11.
The human kallikrein-related peptidases (KLK) are considered important prognostic biomarkers in cancer. The aim of the current study is to demonstrate gene expression of KLK7 in colorectal cancer (CRC) and to correlate the relative KLK7 expression level with clinicopathological factors of CRC.
KLK7 messenger RNA (mRNA) expression was examined in nine CRC cancer cell lines by real-time polymerase chain reaction. The expression levels of KLK7 mRNA in cancerous tissues (n = 136) and paired normal tissues (n = 136) of CRC patients were also examined.
Six of the nine cell lines expressed the KLK7 gene. KLK7 mRNA expression levels in cancer tissues were significantly higher than those in normal tissues. Multivariate analysis revealed that the KLK7 mRNA expression level in cancer was an independent prognostic factor, especially in liver metastasis.
We provide evidence suggesting that KLK7 mRNA expression is correlated with prognosis in CRC patients, especially in liver metastasis.
人激肽释放酶相关肽酶（KLK）被认为是癌症重要的预后生物标志物。本研究旨在证实KLK7在结直肠癌（CRC）中的基因表达，并将相对KLK7表达水平与CRC的临床病理因素相关联。
通过实时聚合酶链反应检测9种CRC癌细胞系中KLK7信使核糖核酸（mRNA）的表达。还检测了CRC患者癌组织（n = 136）和配对正常组织（n = 136）中KLK7 mRNA的表达水平。
9种细胞系中有6种表达KLK7基因。癌组织中KLK7 mRNA表达水平显著高于正常组织。多变量分析显示，癌组织中KLK7 mRNA表达水平是一个独立的预后因素，尤其是在肝转移方面。
我们提供的证据表明，KLK7 mRNA表达与CRC患者的预后相关，尤其是在肝转移方面。
