Department of Neuroscience and Pharmacology, Section of Neuroprotection, Panum Institute, University of Copenhagen, Denmark.
Glia. 2010 Aug;58(10):1220-7. doi: 10.1002/glia.21002.
Neuroglobin (Ngb) is proposed to be a neuron-specific, hypoxia-responsive, neuroprotective protein. However, results are conflicting concerning both Ngb's physiological and pathological significance. This study was designed to investigate the in vivo localization and regulation of Ngb in different neuropathological models representing traumatic injury, infectious, autoimmune, and excitotoxic pathogeneses. We profiled Ngb immunohistochemistry in murine models of traumatic brain injury, cerebral malaria, experimental autoimmune encephalitis, and kainic acid (KA)-mediated epileptic seizures that, to our knowledge, have not been studied in the context of Ngb. In control mice Ngb was expressed exclusively in neurons. In all pathological models except KA, in addition to neurons Ngb was present in reactive astrocytes. Ngb positive astrocytes were found within regions associated with most severe pathology and the astroglial scar. This is the first report of Ngb present in reactive astroglia and in scar-forming astrocytes in response to different pathological conditions relevant to human disease. In light of previously reported cyto-protective properties of Ngb, further insight may result in therapeutic ramifications.
神经球蛋白（Ngb）被认为是一种神经元特异性、缺氧反应性、神经保护蛋白。然而，关于 Ngb 的生理和病理意义，结果存在矛盾。本研究旨在研究不同神经病理学模型中 Ngb 的体内定位和调节，这些模型代表创伤性损伤、感染、自身免疫和兴奋毒性发病机制。我们对创伤性脑损伤、脑疟疾、实验性自身免疫性脑炎和海人酸（KA）介导的癫痫发作的小鼠模型进行了 Ngb 免疫组织化学分析，据我们所知，这些模型在 Ngb 背景下尚未进行研究。在对照小鼠中，Ngb 仅在神经元中表达。在除 KA 以外的所有病理模型中，除神经元外，Ngb 还存在于反应性星形胶质细胞中。在与人类疾病相关的不同病理条件下，Ngb 阳性星形胶质细胞存在于与最严重病理和星形胶质瘢痕相关的区域中。这是首次报道 Ngb 存在于反应性星形胶质细胞和形成瘢痕的星形胶质细胞中，以应对不同的病理条件。鉴于先前报道的 Ngb 的细胞保护特性，进一步的研究可能会产生治疗上的影响。
