Volgushchev S A, Kosykh V A, Podrez E A, Novikova D K, Karpov R S, Repin V S
Biull Eksp Biol Med. 1991 Mar;111(3):250-1.
Effects of pure and long-stored commercial cholesterol feeding on rabbit plasma cholesterol level, on rabbit hepatocyte cholesterol esters levels, and on receptor activity of rabbit hepatocytes were investigated in three experimental groups. In comparison with control, the cholesterol levels in plasma of rabbits, fed with pure and long-stored cholesterol, were 3 and 15 times higher accordingly. Free cholesterol and cholesterol esters concentrations were enhanced in hepatocytes of rabbits fed with pure cholesterol (1.4 and 2.3 times, accordingly, p 0.05) and much more enhanced in hepatocytes of rabbits fed with long-stored cholesterol (4.5 and 24 times, accordingly, p less than 0.05). Specific binding of 125-1-labeled VLDL and LDL to rabbit hepatocytes was decreased in experimental groups by 20% and 32%, accordingly in the first group and by 40% and 77%, accordingly in the second group.
在三个实验组中研究了纯胆固醇和长期储存的商用胆固醇喂养对兔血浆胆固醇水平、兔肝细胞胆固醇酯水平以及兔肝细胞受体活性的影响。与对照组相比，喂食纯胆固醇和长期储存胆固醇的兔子血浆中的胆固醇水平分别高出3倍和15倍。喂食纯胆固醇的兔子肝细胞中游离胆固醇和胆固醇酯浓度相应提高（分别为1.4倍和2.3倍，p<0.05），而喂食长期储存胆固醇的兔子肝细胞中浓度提高得更多（分别为4.5倍和24倍，p<0.05）。在实验组中，125-I标记的极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）与兔肝细胞的特异性结合相应减少，第一组分别减少20%和32%，第二组分别减少40%和77%。
