Hu Yan-jie, Qiu Yuan-hua, Chen En-guo, Ying Ke-jing, Yu Jin, Wang Ping
Department of Pulmonary Medicine, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 May;39(3):278-84. doi: 10.3785/j.issn.1008-9292.2010.03.011.
To identify the volatile organic compounds (VOCs) in lung cancer tissue and lung cancer cell lines.
The lung cancer tissue samples from 18 patients were cultured and 4 lung cell lines (A549, NCI-H446, SK-MES-1, BEAS-2B) were also included in the study. Air samples in the headspace of culture flasks were analyzed for VOCs with solid-phase micro-extraction and gas chromatography-mass spectroscopy technique (SPME-GC/MS).
Two kinds of VOCs 2-pentadecanone and nonadecane were detected in lung cancer cell lines A549, NCI-H446 and SK-MES-1. The concentration of 2-pentadecanone were (1.382 + or -0.171) X 10(-5)mg/L, (1.681 + or - 0.190) X 10(-4)mg/L and (2.835 + or - 0.401) X 10(-6)mg/L, respectively; the concentrations of nonadecane were (8.382 + or - 0.606 ) X 10(-6)mg/L, (1.845 + or - 0.130) X 10(-5)mg/L and (6.220 + or - 0.362) X 10(-6)mg/L), respectively. The eicosane was detected in A549 and NCI-H446 with the concentration of (8.313 + or - 1.130) X 10(-6)mg/L and (1.020 + or - 0.141) X 10(-5)mg/L), respectively. All the 3 VOCs were not detected in cell line BEAS-2B. The concentrations of 12 VOCs including decane, 2- pentadecanone, nonadecane and eicosane were high in 18 lung cancer tissue samples; the concentrations of 2-pentadecanone were 5.421 X 10(-6)mg/L-3.621 X 10(-5)mg/L,those of nonadecane were 5.805 X 10(-6)mg/L-1.830 X 10(-5)mg/L, those of eicosane were 2.730 X 10(-6)mg/L-2.343 X 10(-5)mg/L. There were no differences of VOCs levels among patients with different cancer differentiation (P>0.05). The concentration of eicosane in the non-squamous carcinoma was higher than that in squamous carcinoma, the same results were confirmed in the lung cancer cell lines.
This study has identified VOCs produced by lung cancer tissue, which may support to use breath test as a complementary noninvasive diagnostic method for lung cancer.
鉴定肺癌组织及肺癌细胞系中的挥发性有机化合物（VOCs）。
培养18例患者的肺癌组织样本，并将4种肺癌细胞系（A549、NCI-H446、SK-MES-1、BEAS-2B）纳入研究。采用固相微萃取和气相色谱-质谱联用技术（SPME-GC/MS）分析培养瓶顶空的空气样本中的VOCs。
在肺癌细胞系A549、NCI-H446和SK-MES-1中检测到两种VOCs，即2-十五烷酮和十九烷。2-十五烷酮的浓度分别为（1.382±0.171）×10⁻⁵mg/L、（1.681±0.190）×10⁻⁴mg/L和（2.835±0.401）×10⁻⁶mg/L；十九烷的浓度分别为（8.382±0.606）×10⁻⁶mg/L、（1.845±0.130）×10⁻⁵mg/L和（6.220±0.362）×10⁻⁶mg/L。在A549和NCI-H446中检测到二十烷，浓度分别为（8.313±1.130）×10⁻⁶mg/L和（1.020±0.141）×10⁻⁵mg/L。在细胞系BEAS-2B中未检测到这3种VOCs。18例肺癌组织样本中包括癸烷、2-十五烷酮、十九烷和二十烷在内的12种VOCs浓度较高；2-十五烷酮的浓度为5.421×10⁻⁶mg/L - 3.621×10⁻⁵mg/L，十九烷的浓度为5.805×10⁻⁶mg/L - 1.830×10⁻⁵mg/L，二十烷的浓度为2.730×10⁻⁶mg/L - 2.343×10⁻⁵mg/L。不同癌症分化程度患者的VOCs水平无差异（P>0.05）。非鳞癌中二十烷的浓度高于鳞癌，在肺癌细胞系中也得到同样结果。
本研究鉴定出肺癌组织产生的VOCs，这可能支持将呼气试验作为肺癌的一种辅助非侵入性诊断方法。
