Wang Heng, Song Liangke, Tang Hao, He Haiyang, Dai Chunchu, Li Xiaofeng
Life Science and Engineering College of Southwest Jiaotong University, Chengdu 610031, China.
Zhongguo Zhong Yao Za Zhi. 2010 Mar;35(6):669-72. doi: 10.4268/cjcmm20100601.
To screen the representing components in different germplasms of Canarium album and determine the content by HPLC for laying the foundation for the quality specification.
Gallic acid and scopoletin and scoparone were chosen as the main chemical components for clearing heat and relieving sore throat by reviewing literature and qualitative experiment. Gallic acid was determined according the method of Galla chinensis in Chinese pharmacopoeia. Scopoletin and scoparone were determined by HPLC on C18 column (4.6 mm x 250 mm, 5 microm) and the mobile phase was acetonitrile-water-acetic acid (16:84:0.5) with the detection wavelength at 345 nm and column temperature at 35 degrees C , the flow rate was 1.0 mL x min(-1).
The resolution is good for gallic acid and scopoletin and scoparone. The standard curve in detection range showed good linear relation, the average recoveries were 100.22%, 101.22% and 100.42%, respectively, RSD were all less than 2.0%. The content of gallic acid in the large fruit C. album from Guangdong was higher than that in the small fruit C. album, while the result was contrary in samples from Sichuan. The content of total coumarins in the small fruit C. album was generally higher than that in the large fruit C. album, which from Sichuan was higher than those from Guangdong.
The efficacies of clearing heat and relieving sore throat in C. album were formed by synergistic effect of gallic acid and scopoletin and scoparone. The small fruit C. album was better than the large fruit C. album as medicinal materials, the results provide reliable basis for quality control in medicinal materials and its preparation.
筛选橄榄不同种质中的代表性成分，并采用高效液相色谱法测定其含量，为质量标准的制定奠定基础。
通过查阅文献及定性实验，选取没食子酸、东莨菪素和滨蒿内酯作为清热利咽的主要化学成分。没食子酸按照《中国药典》中五倍子的方法测定。东莨菪素和滨蒿内酯采用高效液相色谱法，色谱柱为C18柱（4.6 mm×250 mm，5 µm），流动相为乙腈 - 水 - 乙酸（16∶84∶0.5），检测波长为345 nm，柱温为35℃，流速为1.0 mL·min⁻¹。
没食子酸、东莨菪素和滨蒿内酯分离度良好。检测范围内标准曲线线性关系良好，平均回收率分别为100.22%、101.22%和100.42%，相对标准偏差均小于2.0%。广东大果型橄榄中没食子酸含量高于小果型橄榄，而四川样品结果相反。小果型橄榄中总香豆素含量总体高于大果型橄榄，其中四川的高于广东的。
橄榄清热利咽功效是没食子酸、东莨菪素和滨蒿内酯协同作用的结果。小果型橄榄作为药材优于大果型橄榄，研究结果为药材及其制剂的质量控制提供了可靠依据。
