[板蓝根中表告依春的含量测定及其反相高效液相色谱法制备]
[Content determination of epigoitrin in Radix Isatidis and its preparation by RP-HPLC].
作者信息
An Yi-Qiang, Jia Xiao-Bin, Yuan Hai-Jian, Sun E, Xu Zhen-Zhen
机构信息
Jiangsu Provincial Key Laboratory of New Drug Delivery System of Chinese Materia Medica & Key Laboratory of Scientific Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine, Nanjing 210028, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Sep;33(18):2074-6.
OBJECTIVE
To establish an HPLC method for the content determination of epigoitrin in Radix Isatidis and its preparation, and to provide valuable data for quality control of Radix Isatidis and its preparation.
METHOD
The samples were separated on a ZORBAX SB-C18 (4. 6 mm x 150 mm, 5 microm) column with the mobile phase of acetonitrile-water-phosphoric acid-triethylamine (8.50 : 90.72 : 0.73 : 0.05) in the flow rate of 0.7 mL x min(-1). The detection wavelength was set at 245 nm. Column temperature was 30 degrees C.
RESULT
The linear range of epigoitrin was 0.0204-0.3060 microg (r = 0.9998), and the average recovery was 98.99% with the RSD was 1.31% (n = 9).
CONCLUSION
The method for quantitation of epigoitrin in Radix Isatidis and its preparation was accurate and reliable, which can be used to evaluate the quality of Radix Isatidis and its preparation.
目的
建立高效液相色谱法测定板蓝根及其制剂中表告依春的含量，为板蓝根及其制剂的质量控制提供有价值的数据。
方法
采用ZORBAX SB-C18（4.6 mm×150 mm，5μm）色谱柱，以乙腈-水-磷酸-三乙胺（8.50∶90.72∶0.73∶0.05）为流动相，流速为0.7 mL·min-1，检测波长为245 nm，柱温为30℃。
结果
表告依春的线性范围为0.0204～0.3060μg（r = 0.9998），平均回收率为98.99%，相对标准偏差为1.31%（n = 9）。
结论
该方法测定板蓝根及其制剂中表告依春的含量准确可靠，可用于评价板蓝根及其制剂的质量。
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