[益气养阴活血通络中药对早期糖尿病大鼠肾脏p38丝裂原活化蛋白激酶信号通路的影响]
[Effect of supplementing Qi-nourishing Yin-dispersing blood stasis-dredging collateral herbs on p38 MAPK signaling pathway in kidney of early diabetic rats].
作者信息
Zhao Wenhong, Chen Zhiqiang, Zhang Jianghua, Sun Yufeng, Wang Yuehua, Wang Huiqing
机构信息
Urology Department, Hospital of Chinese Medicine of Hebei Medical University, Shijiazhuang 050011, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Mar;35(6):768-71. doi: 10.4268/cjcmm20100624.
OBJECTIVE
To study the effect of supplementing Qi-nourishing Yin and dispersing blood stasis-dredging collateral herbs on p38 mitogen activated protein kinase (p38 MAPK) signaling pathway in the kidney of early diabetic rats.
METHOD
Dividing SD rats randomly into 6 groups: Simple nephrectomy group, model group, irbesatan group, traditional Chinese medicine (TCM) low dose group, TCM middle dose group and TCM high dose group. Each group of rats was fed with the corresponding dose of medicine. After 6 weeks, detecting 24 h urine protein (UPro) level, renal function, p38 MAPK mRNA and p-p38 MAPK protein level.
RESULT
UPro levels of irbesatan group, TCM low group and TCM middle dose group decreased significantly (P < 0.05) , compared with that of the model group. Renal function of the treated groups was improved greatly and their p38 MAPK mRNA and p-p38MAPK protein levels decreased significantly (P < 0.05), compared with those of the model group.
CONCLUSION
Supplementing Qi-nourishing Yin-dispersing blood stasis-dredging collateral herbs could treat DN rats effectively by inhibiting the expression of p38 MAPK signaling pathway.
目的
研究益气养阴活血通络中药对早期糖尿病大鼠肾脏p38丝裂原活化蛋白激酶（p38 MAPK）信号通路的影响。
方法
将SD大鼠随机分为6组：单纯假手术组、模型组、厄贝沙坦组、中药低剂量组、中药中剂量组和中药高剂量组。每组大鼠给予相应剂量药物。6周后，检测24小时尿蛋白（UPro）水平、肾功能、p38 MAPK mRNA及p-p38 MAPK蛋白水平。
结果
与模型组比较，厄贝沙坦组、中药低剂量组和中药中剂量组UPro水平显著降低（P<0.05）。各治疗组大鼠肾功能明显改善，p38 MAPK mRNA及p-p38 MAPK蛋白水平显著降低（P<0.05）。
结论
益气养阴活血通络中药可通过抑制p38 MAPK信号通路表达有效治疗糖尿病肾病大鼠。
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