Zhou Xuemei, Xu Congshu, Wang Kai, Chu Quangen, Dong Changwu, Wu Chuanyun, Zhao Jiangen, Li Lingxiu, Wang Li
College of Traditional Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Jul 30;39(7):855-860. doi: 10.12122/j.issn.1673-4254.2019.07.17.
To observe the effects of a traditional Chinese medicine (TCM) capsule for replenishing qi, nourishing yin and activating blood on Notch/Hes1 signaling pathway in the renal tissue and vascular endothelial CD34 and CD144 expressions in a rat model of diabetic nephropathy.
Rat models of early-stage diabetic nephropathy were established by left nephrectomy and high- fat and high- sugar feeding combined with intraperitoneal injection of STZ. The rats were randomized into model group, benazepril group, and high-, moderate-, and low-dose TCM capsule groups for corresponding treatments, with 6 normal rats as the control group. After 8 weeks of drug treatment, blood glucose and 24-h urinary albumin of the rats were measured, and the renal histopathology was observed with HE staining; Hes1 expression in the renal tissue was detected with immunohistochemical staining, and the renal expressions of CD34 and CD144 were detected using Western blotting.
Compared with the normal control group, the rat models of diabetic nephropathy showed obvious abnormalities in 24- h urinary albumin and expressions of Hes1, CD34 and CD144d. The TCM capsule at both the high and moderate doses significantly reduced 24-h urinary albumin in the rats; the renal expressions of Hes1 and CD34 was significantly reduced in all the dose groups, and the expression of CD144 was significantly reduced in the high- dose group. Compared with benazepril group, the TCM capsule obviously reduced CD34 expression at all the 3 doses and lowered CD144 expression at the low dose. Histopathologically, the rats in the model group showed glomerular hypertrophy, increased mesenteric matrix, thickening and widening of the mesenteric membrane, and nodular hyperplasia. These pathologies were obviously alleviated by treatment with the TCM capsule at the high and moderate doses.
The Traditional Chinese medicine (TCM) capsule for replenishing qi, nourishing yin and activating blood can reduce Hes1, CD34 and CD144 in kidney tissue of model rats, play a protective role on kidney function and delay the development of DN.
观察益气养阴活血中药胶囊对糖尿病肾病大鼠模型肾组织Notch/Hes1信号通路及血管内皮CD34和CD144表达的影响。
采用左肾切除、高脂高糖喂养并腹腔注射链脲佐菌素建立早期糖尿病肾病大鼠模型。将大鼠随机分为模型组、苯那普利组、中药胶囊高、中、低剂量组进行相应处理，另设6只正常大鼠作为对照组。药物治疗8周后，检测大鼠血糖和24小时尿白蛋白，HE染色观察肾组织病理学变化；免疫组化染色检测肾组织中Hes1表达，Western blotting检测肾组织中CD34和CD144表达。
与正常对照组相比，糖尿病肾病大鼠模型24小时尿白蛋白及Hes1、CD34和CD144表达明显异常。中药胶囊高、中剂量组均能显著降低大鼠24小时尿白蛋白；各剂量组肾组织中Hes1和CD34表达均显著降低，高剂量组CD144表达显著降低。与苯那普利组相比，中药胶囊3个剂量组均明显降低CD34表达，低剂量组降低CD144表达。组织病理学上，模型组大鼠表现为肾小球肥大、系膜基质增多、系膜膜增厚增宽及结节样增生。高、中剂量中药胶囊治疗明显减轻了这些病变。
益气养阴活血中药胶囊可降低模型大鼠肾组织中Hes1、CD34和CD144表达，对肾功能起到保护作用，延缓糖尿病肾病的发展。
