Rağbetli Murat C, Aydinlioğlu Atif, Koyun Necat, Rağbetli Cennet, Bektas Seyman, Ozdemir Serdar
Department of Histology and Embryology, Medical Faculty, Yüzüncü Yil University, Van, Turkey.
Int J Radiat Biol. 2010 Jul;86(7):548-54. doi: 10.3109/09553001003734527.
The World Health Organisation proposed an investigation concerning the exposure of animals to radiofrequency fields because of the possible risk factor for health. At power frequencies there is evidence to associate both childhood leukaemia and brain tumours with magnetic field exposures. There is also evidence of the effect of mobile phone exposure on both cognitive functions and the cerebellum. Purkinje cells of the cerebellum are also sensitive to high dose microwave exposure in rats. The present study investigated the effect of exposure to mobile phone on the number of Purkinje and granule neurons in the developing cerebellum.
Male and female Swiss albino mice were housed as control and mobile phone-exposed groups. Pregnant animals in the experimental group were exposed to Global System for Mobile Communication (GSM) mobile phone radiation at 890-915 MHz at 0.95 W/Kg specific absorption rate (SAR). The cerebella were processed by frozen microtome. The sections obtained were stained with Haematoxylin-eosin and cresyl violet. For cell counting by the optical fractionator method, a pilot study was firstly performed. Cerebellar areas were analysed by using Axiovision software running on a personal computer. The optical dissectors were systematically spaced at random, and focused to the widest profile of the neuron cell nucleus.
A significant decrease in the number of Purkinje cells and a tendency for granule cells to increase in cerebellum was found.
Further studies in this area are needed due to the popular use of mobile telephones and relatively high exposure on developing brain.
世界卫生组织提议对动物暴露于射频场的情况展开调查，因为这可能是一个健康风险因素。在工频下，有证据表明儿童白血病和脑肿瘤都与磁场暴露有关。也有证据显示手机暴露对认知功能和小脑均有影响。大鼠小脑的浦肯野细胞对高剂量微波暴露也很敏感。本研究调查了手机暴露对发育中小脑浦肯野神经元和颗粒神经元数量的影响。
将雄性和雌性瑞士白化小鼠分为对照组和手机暴露组饲养。实验组的怀孕动物暴露于全球移动通信系统（GSM）手机辐射下，频率为890 - 915 MHz，比吸收率（SAR）为0.95 W/Kg。小脑用冷冻切片机处理。得到的切片用苏木精 - 伊红和甲酚紫染色。为采用光学分割法进行细胞计数，首先进行了一项预实验。使用运行在个人计算机上的Axiovision软件分析小脑区域。光学分割器随机系统地间隔排列，并聚焦于神经元细胞核的最宽轮廓。
发现小脑中浦肯野细胞数量显著减少，颗粒细胞数量有增加的趋势。
鉴于手机的广泛使用以及对发育中大脑的相对高暴露，该领域需要进一步研究。
