Neurobiology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, India.
School of Life and Basic Sciences, Jaipur National University, Jaipur, Rajasthan, India.
Mol Cell Biochem. 2017 Nov;435(1-2):1-13. doi: 10.1007/s11010-017-3051-8. Epub 2017 May 3.
For decades, there has been an increasing concern about the potential hazards of non-ionizing electromagnetic fields that are present in the environment and alarming as a major pollutant or electro-pollutant for health risk and neuronal diseases. Therefore, the objective of the present study was to explore the effects of 10 GHz microwave radiation on developing mice brain. Two weeks old mice were selected and divided into two groups (i) sham-exposed and (ii) microwave-exposed groups. Animals were exposed for 2 h/day for 15 consecutive days. After the completion of exposure, within an hour, half of the animals were autopsied immediately and others were allowed to attain 6 weeks of age for the follow-up study. Thereafter results were recorded in terms of various biochemical, behavioral, and histopathological parameters. Body weight result showed significant changes immediately after treatment, whereas non-significant changes were observed in mice attaining 6 weeks of age. Several other endpoints like brain weight, lipid peroxidation, glutathione, protein, catalase, and superoxide dismutase were also found significantly (p < 0.05) altered in mice whole brain. These significant differences were found immediately after exposure and also in follow-up on attaining 6 weeks of age in microwave exposure group. Moreover, statistically significant (p < 0.001) effect was investigated in spatial memory of the animals, in learning to locate the position of platform in Morris water maze test. Although in probe trial test, sham-exposed animals spent more time in searching for platform into the target quadrant than in opposite or other quadrants. Significant alteration in histopathological parameters (qualitative and quantitative) was also observed in CA1 region of the hippocampus, cerebral cortex, and ansiform lobule of cerebellum. Results from the present study concludes that the brain of 2 weeks aged mice was very sensitive to microwave exposure as observed immediately after exposure and during follow-up study at 6 weeks of age.
几十年来，人们越来越关注环境中存在的非电离电磁场的潜在危害，这些电磁场是主要的污染物或电污染物，对健康风险和神经疾病构成威胁。因此，本研究的目的是探讨 10GHz 微波辐射对发育中老鼠大脑的影响。选择两周大的老鼠，并将其分为两组：（i）假暴露组和（ii）微波暴露组。动物每天暴露 2 小时，连续 15 天。暴露完成后，在一小时内，一半的动物立即进行尸检，另一半动物则允许达到 6 周龄进行后续研究。此后，根据各种生化、行为和组织病理学参数记录结果。体重结果显示，治疗后立即发生显著变化，而达到 6 周龄的老鼠则无明显变化。其他几个终点，如大脑重量、脂质过氧化、谷胱甘肽、蛋白质、过氧化氢酶和超氧化物歧化酶，在整个老鼠大脑中也发现有显著（p<0.05）改变。这些显著差异在暴露后立即发现，并且在达到 6 周龄的后续研究中也发现微波暴露组有显著差异。此外，还对动物的空间记忆进行了统计学上的显著（p<0.001）影响研究，在 Morris 水迷宫测试中，学习定位平台的位置。虽然在探针试验中，假暴露组的动物在搜索目标象限的平台时花费的时间比在相反象限或其他象限多。在海马 CA1 区、大脑皮层和小脑ansiform 叶中也观察到组织病理学参数（定性和定量）的显著改变。本研究的结果表明，2 周龄老鼠的大脑对微波暴露非常敏感，这一观察结果不仅在暴露后立即出现，而且在 6 周龄的后续研究中也出现。
