Naderi Mostafa, Jadidi Khosro, Falahati Farzaneh, Alavi Saayyed Ali
Department of Ophthalmology, Baghiatallah University of Medical Sciences, Isfahan, Iran.
Cutan Ocul Toxicol. 2010 Dec;29(4):234-40. doi: 10.3109/15569527.2010.491102. Epub 2010 Jun 15.
Sulfur mustard (SM) is an alkylating agent that can affect cornea and induce various complications. With regard to the role of the enzyme collagenase in dermatologic complications induced by sm and its role in other ocular disorders, we studied the effect of SM and nitrogen mustard (NM) on collagen degradation by collagenase.
This study included 7 groups of samples: The negative control group contained collagen without collagenase and toxins, the control group contained collagen and collagenase without any toxin, the positive control groups of NM and SM contained collagen and NM or SM without collagenase, the experimental groups of NM and SM contained collagen that was affected by NM or SM and collagenase, and the control group of collagenase contained only collagenase without containing collagen or receiving toxins. After incubation for 3.5 hours, the amount of hydroxyproline and the protein content of the samples were measured. Data were analyzed by analysis of variance (ANOVA).
The protein concentrations of the negative control group and the positive control groups of SM and NM were significantly lower than those for all other groups of the study. There was a significant difference in hydroxyproline concentration of control group and negative control group; however, there was no significant difference between experimental group of SM and the positive control group of SM. There was no significant difference between the negative control group and the positive control group of SM in the hydroxyproline concentration of sediment samples.
According to the results of this study, SM can affect the corneal collagen in a way in which collagenase cannot degrade it. In addition, it can be hypothesized that ineffective activity of this enzyme can result in increasing concentration of collagenase, which can lead to the destruction of the normal collagen of the cornea. The main result of this study confirms the hypothesis that SM inhibits the effect of collagenase on corneal collagen.
硫芥（SM）是一种烷化剂，可影响角膜并引发各种并发症。关于胶原酶在SM所致皮肤并发症中的作用及其在其他眼部疾病中的作用，我们研究了SM和氮芥（NM）对胶原酶降解胶原蛋白的影响。
本研究包括7组样本：阴性对照组包含不含胶原酶和毒素的胶原蛋白，对照组包含含胶原酶但无任何毒素的胶原蛋白，NM和SM阳性对照组包含含NM或SM但不含胶原酶的胶原蛋白，NM和SM实验组包含受NM或SM影响的胶原蛋白及胶原酶，胶原酶对照组仅含胶原酶，不含胶原蛋白或未接触毒素。孵育3.5小时后，测量样本中羟脯氨酸含量和蛋白质含量。数据采用方差分析（ANOVA）进行分析。
阴性对照组以及SM和NM阳性对照组的蛋白质浓度显著低于本研究的所有其他组。对照组和阴性对照组的羟脯氨酸浓度存在显著差异；然而，SM实验组与SM阳性对照组之间无显著差异。沉淀样本的羟脯氨酸浓度在SM阴性对照组和阳性对照组之间无显著差异。
根据本研究结果，SM可通过胶原酶无法降解的方式影响角膜胶原蛋白。此外，可以推测该酶的无效活性会导致胶原酶浓度升高，进而导致角膜正常胶原蛋白的破坏。本研究的主要结果证实了SM抑制胶原酶对角膜胶原蛋白作用的假设。
