Department of Periodontology and Endodontology, Division of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Kita-ku, Sapporo, Japan.
J Periodontal Res. 2010 Oct;45(5):658-63. doi: 10.1111/j.1600-0765.2010.01281.x. Epub 2010 Jun 10.
Modification of the root surface may play an important role in regenerating the periodontal attachment between the root and periodontal connective tissue. We speculated that bone morphogenetic protein (BMP) application to the root surface constructed a novel attachment by cementum-like hard tissue, although gingival connective tissue proliferated to the root surface. The aim of this study was to examine whether BMP-2 guided cementum-like tissue deposition on a BMP-conditioned root surface.
Root dentin on the buccal side of 24 teeth in four beagle dogs was surgically exposed. The denuded root dentin surfaces were demineralized with EDTA and washed with saline. Subsequently, 15 microL of BMP-2 solution (loading dose, 0.4 and 1.0 microg/microL) was applied to the root dentin surface. In the control roots, phosphate-buffered saline was applied to the root surface. Specimens were analyzed histologically 16 wk after surgery.
Formation of cementum-like tissue was frequently observed on the BMP-2-conditioned root at the coronal portion. Cellular cementum-like tissue was separated from the original cementum and encapsulated with gingival connective tissue. Cementum-like tissue formation with BMP-2 at 1.0 microg/microL was significantly greater than that in the control roots and those with BMP-2 at 0.4 microg/microL. Downgrowth of the junctional epithelium in the 1.0 microg/microL BMP-2 group was significantly less than that in the control roots.
Root dentin surface conditioning with BMP-2 stimulated cementum-like tissue formation and inhibited epithelial downgrowth.
对牙根表面的改性可能在根与牙周结缔组织之间再生牙周附着中发挥重要作用。我们推测，骨形态发生蛋白（BMP）应用于牙根表面可通过类似于牙骨质的硬组织构建新的附着，尽管牙龈结缔组织向牙根表面增殖。本研究旨在探讨 BMP-2 是否能引导 BMP 处理后的牙根表面形成类似于牙骨质的组织。
在四只比格犬的 24 颗牙齿的颊侧进行了牙根表面手术暴露。用 EDTA 脱矿并用生理盐水冲洗暴露的牙根牙本质表面。随后，将 15 μL 的 BMP-2 溶液（加载剂量为 0.4 和 1.0 μg/μL）应用于牙根牙本质表面。在对照牙根中，将磷酸盐缓冲盐水应用于牙根表面。术后 16 周进行标本组织学分析。
在 BMP-2 处理的牙根冠部，经常观察到类似于牙骨质的组织形成。细胞性类似于牙骨质的组织与原有的牙骨质分离，并被牙龈结缔组织包裹。在 1.0 μg/μL 的 BMP-2 处理组中，类似于牙骨质的组织形成明显大于对照组和 0.4 μg/μL 的 BMP-2 处理组。1.0 μg/μL 的 BMP-2 组中，结合上皮的向下生长明显少于对照组。
BMP-2 处理牙根表面可刺激类似于牙骨质的组织形成，并抑制上皮向下生长。
