Department of Periodontology and Endodontology, Division of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
J Periodontal Res. 2012 Oct;47(5):626-34. doi: 10.1111/j.1600-0765.2012.01475.x. Epub 2012 Mar 23.
A three-dimensional scaffold may play an important role in periodontal tissue engineering. We prepared bio-safe collagen hydrogel, which exhibits properties similar to those of native extracellular matrix. The aim of this study was to examine the effect of implantation of collagen hydrogel/sponge scaffold on periodontal wound healing in class II furcation defects in dogs.
The collagen hydrogel/sponge scaffold was prepared by injecting collagen hydrogel, cross-linked to the ascorbate-copper ion system, into a collagen sponge. Class II furcation defects (of 5 mm depth and 3 mm width) were surgically created in beagle dogs. The exposed root surface was planed and demineralized with EDTA. In the experimental group, the defect was filled with collagen hydrogel/sponge scaffold. In the control group, no implantation was performed. Histometric parameters were evaluated 2 and 4 wk after surgery.
At 2 wk, the collagen hydrogel/sponge scaffold displayed high biocompatibility and biodegradability with numerous cells infiltrating the scaffold. In the experimental group, reconstruction of alveolar bone and cementum was frequently observed 4 wk after surgery. Periodontal ligament tissue was also re-established between alveolar bone and cementum. Volumes of new bone, new cementum and new periodontal ligament were significantly greater in the experimental group than in the control group. In addition, epithelial down-growth was suppressed by application of collagen hydrogel.
The collagen hydrogel/sponge scaffold possessed high tissue compatibility and degradability. Implantation of the scaffold facilitated periodontal wound healing in class II furcation defects in beagle dogs.
三维支架可能在牙周组织工程中发挥重要作用。我们制备了生物安全的胶原水凝胶，其表现出与天然细胞外基质相似的特性。本研究旨在观察胶原水凝胶/海绵支架植入对犬类 II 类分叉缺损牙周创面愈合的影响。
通过将胶原水凝胶注入到胶原海绵中，交联到抗坏血酸-铜离子系统中，制备胶原水凝胶/海绵支架。在比格犬中，通过手术创建 II 类分叉缺损（深度为 5mm，宽度为 3mm）。暴露的根面进行平整和 EDTA 脱矿处理。在实验组中，用胶原水凝胶/海绵支架填充缺损。在对照组中，不进行植入。术后 2 周和 4 周评估组织学参数。
在第 2 周时，胶原水凝胶/海绵支架显示出良好的生物相容性和可降解性，有大量细胞浸润支架。在实验组中，术后 4 周时经常观察到牙槽骨和牙骨质的重建。牙周韧带组织也在牙槽骨和牙骨质之间重新建立。实验组的新骨、新牙骨质和新牙周韧带的体积明显大于对照组。此外，胶原水凝胶的应用抑制了上皮向下生长。
胶原水凝胶/海绵支架具有较高的组织相容性和可降解性。支架的植入促进了比格犬 II 类分叉缺损的牙周创面愈合。
