Ripamonti U, Heliotis M, Rueger D C, Sampath T K
Bone Research Laboratory, Medical Research Council/University of the Witwatersrand, Johannesburg, South Africa.
Arch Oral Biol. 1996 Jan;41(1):121-26. doi: 10.1016/0003-9969(95)00110-7.
Recombinant human osteogenic protein-1 (hOP-1), a member of the bone morphogenetic protein family, was examined for its efficacy in periodontal regeneration. Twelve furcation defects, surgically prepared in the first and second mandibular molars, were treated with bovine insoluble collagenous matrix in conjunction with 0.0 (control), 100 and 500 mu g of recombinant hOP-1 per g of matrix. After 60 days of healing, histological and histometric analyses on serial, undemineralized sections cut at 7 mu m showed substantial cementogenesis on the exposed dentine of furcations treated with both doses of hOP-1 (p < 0.01 vs control). Foci of nascent mineralization were seen within the newly deposited cementoid along the coronal areas of hOP-1-treated defects. Within the furcations, there were substantial amounts of residual collagenous carrier, interspersed with a mineralized matrix having histological features of cementum. This mineralized cementum-like material was predominantly deposited around the carrier, and blended into newly formed cementum along the root surfaces. In the apical area, the cementum-like material and the remaining alveolar bony housing were not connected; indeed the two components were separated by a fibrovascular tissue that had numerous features of the periodontal ligament space. Formation and insertion of Sharpey's fibres into newly formed root cementum were also observed. It is likely that the expression of specific cell phenotypes by hOP-1 is regulated, in part, by the extracellular matrix microenvironment, including dentine. Thus, exposed dentine, in the presence of exogenous hOP-1 at the doses tested, may preferentially modulate the expression of the cementogenic phenotype. These findings in a non-human primate show that hOP-1, at the doses tested, induced cementogenesis on surgically denuded root surfaces, indicating a specific function during repair and regeneration of periodontal tissues.
重组人骨生成蛋白-1（hOP-1）是骨形态发生蛋白家族的一员，对其在牙周再生中的功效进行了检测。在下颌第一和第二磨牙上通过手术制备了12个根分叉缺损，用牛不溶性胶原基质结合每克基质0.0（对照）、100和500μg的重组hOP-1进行治疗。愈合60天后，对7μm厚的连续未脱钙切片进行组织学和组织计量学分析，结果显示，用两种剂量的hOP-1处理的根分叉暴露牙本质上有大量牙骨质生成（与对照相比，p<0.01）。在hOP-1处理缺损的冠部区域，新沉积的类牙骨质内可见新生矿化灶。在根分叉内，有大量残留的胶原载体，其间夹杂着具有牙骨质组织学特征的矿化基质。这种矿化的类牙骨质物质主要沉积在载体周围，并沿根面融入新形成的牙骨质中。在根尖区域，类牙骨质物质与剩余的牙槽骨包壳未相连；实际上，这两个成分被具有牙周膜间隙诸多特征的纤维血管组织分隔开。还观察到沙比纤维形成并插入新形成的牙根牙骨质中。hOP-1对特定细胞表型的表达可能部分受细胞外基质微环境（包括牙本质）的调节。因此，在所测试剂量的外源性hOP-1存在下，暴露的牙本质可能优先调节牙骨质生成表型的表达。在非人类灵长类动物中的这些发现表明，在所测试的剂量下，hOP-1可在手术暴露的根面上诱导牙骨质生成，表明其在牙周组织修复和再生过程中具有特定功能。
