Department of Pharmacology and Neuroscience Program, Zebrafish Neuroscience Research Consortium (ZNRC), Tulane University Medical School, 1430 Tulane Ave., New Orleans, LA 70112, USA.
Brain Res. 2010 Aug 12;1348:209-15. doi: 10.1016/j.brainres.2010.06.012. Epub 2010 Jun 12.
Larval zebrafish (Danio rerio) have recently been suggested as a high-throughput experimental model of epilepsy-related pathogenetic states. Here we use adult zebrafish to study behavioral symptoms associated with drug-evoked seizures. Experimental epilepsy-like states were evoked in zebrafish by exposure for 20min to three chemoconvulsant drugs: caffeine (250mg/L; 1.3mM), pentylenetetrazole (1.5g/L; 11.0mM) and picrotoxin (100mg/L; 0.17mM). Fish behavior was analyzed using manual and video-tracking methods (Noldus Ethovision XT7). Compared to their respective controls, all three drug-treated groups showed robust seizure-like responses (hyperactivity bouts, spasms, circular and corkscrew swimming) accompanied by elevated whole-body cortisol levels (assessed by ELISA). In contrast, control fish did not display seizure-like behaviors and had significantly lower cortisol levels. Paralleling behavioral and endocrine phenotypes observed in clinical and rodent studies, our data implicates adult zebrafish as an emerging experimental model for epilepsy research.
幼体斑马鱼（Danio rerio）最近被提议作为一种与癫痫相关的发病状态的高通量实验模型。在这里，我们使用成年斑马鱼来研究与药物诱发的癫痫发作相关的行为症状。通过将斑马鱼暴露于三种化学惊厥药物中 20 分钟，来诱发类似癫痫的实验状态：咖啡因（250mg/L；1.3mM）、戊四氮（1.5g/L；11.0mM）和印防己毒素（100mg/L；0.17mM）。使用手动和视频跟踪方法（Noldus Ethovision XT7）分析鱼类的行为。与各自的对照组相比，所有三种药物处理组均表现出强烈的癫痫样反应（活动过度发作、痉挛、圆形和螺旋形游泳），同时伴有全身皮质醇水平升高（通过 ELISA 评估）。相比之下，对照组鱼没有表现出癫痫样行为，皮质醇水平显著降低。与临床和啮齿动物研究中观察到的行为和内分泌表型相似，我们的数据表明成年斑马鱼是癫痫研究的一种新兴实验模型。
