Ashtown Food Research Centre, Teagasc, Ashtown, Dublin 15, Ireland.
J Microbiol Methods. 2010 Oct;83(1):1-7. doi: 10.1016/j.mimet.2010.06.003. Epub 2010 Jun 11.
The aim of this study was to develop a universal cultural protocol, which could facilitate the growth of 17 species and 3 subspecies of Campylobacter. Enrichment media including Campylobacter Enrichment Broth (CEB) and Bolton Broth were tested against a panel of Campylobacter strains (n=53) encompassing 17 species and 3 subspecies, under a gas atmosphere containing hydrogen (2.5% O(2), 7% H(2), 10% CO(2), and 80.5% N(2)). The impact of enrichment conditions on cell motility was also investigated using fluorescent microscopy. Membrane filtration was examined as a means of selectively recovering Campylobacter from enrichment media on two different non-selective agars, Anaerobe Basal Agar (ABA) and Tryptose Blood Agar (TBA). The results showed that enrichment in CEB for 24 h at 37°C under a modified gas atmosphere followed by centrifugation and membrane filtration onto ABA allowed recovery of all species (53 strains) of Campylobacter from inoculated meat samples. After 24 h enrichment, there were higher levels of motile Campylobacter in CEB than in Bolton broth and it is proposed that this attribute aided the passage of the Campylobacter through the membrane filter. The results of this study provide a simple, but effective method for the growth and recovery of a wide range of diverse Campylobacter spp. from a meat matrix using common cultural parameters.
本研究的目的是开发一种通用的文化方案，以促进 17 种 3 亚种弯曲菌的生长。在包含氢气（2.5%O2、7%H2、10%CO2 和 80.5%N2）的气体环境中，对包括 17 种 3 亚种在内的 53 株弯曲菌菌株进行了包含弯曲菌增菌肉汤（CEB）和 Bolton 肉汤的富集培养基的测试。还使用荧光显微镜研究了富集条件对细胞能动性的影响。通过膜过滤检查了从两种不同的非选择性琼脂（Anaerobe Basal Agar（ABA）和 Tryptose Blood Agar（TBA））的富集培养基中选择性回收弯曲菌的方法。结果表明，在改良气体环境下于 37°C 下在 CEB 中进行 24 小时的富集，然后进行离心和膜过滤到 ABA 上，可从接种的肉样中回收所有弯曲菌（53 株）。经过 24 小时的富集，CEB 中运动性弯曲菌的水平高于 Bolton 肉汤，据推测，这一特性有助于弯曲菌通过膜过滤器。本研究的结果提供了一种简单但有效的方法，可使用常见的培养参数从肉基质中生长和回收广泛的不同弯曲菌属。
