Byun Ji Yeon, Choi Hae Young, Myung Ki Bum, Choi You Won
Department of Dermatology, School of Medicine, Ewha Womans University, Seoul, Korea.
Ann Dermatol. 2009 Feb;21(1):12-7. doi: 10.5021/ad.2009.21.1.12. Epub 2009 Feb 28.
Depending on the light dose and concentration of photosensitizer for photodynamic treatment (PDT), a multitude of dose-related events are demonstrable in PDT-treated cells. Sublethal doses may result in the alteration of cytokine release and consequently modify immune actions, rather than cause cell death.
The purpose of this study was to investigate cytokine expression in cultured HaCaT cells after intense pulse light (IPL) treatment or PDT utilizing 5-aminolevulinic acid (ALA) and IPL at sublethal doses.
Cultured HaCaT cells were treated with either IPL only (4, 8 and 12 J/cm(2)) or ALA-IPL PDT (100micromol/L of ALA; 0, 4, 8, and 12 J/cm(2) of IPL). The expression of IL-10, TGF-beta(1) and TNF-alpha was investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay.
IL-10 protein increased up to 5.95-fold after IPL treatment and up to 2.85-fold after PDT. TGF-beta(1) mRNA and protein showed slight increases after both IPL treatment and PDT, of which the latter induced slightly larger increases. TNF-alpha mRNA and protein showed no induction or reduction after PDT.
Increased expressions of IL-10 and TGF-beta(1) was observed after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses, and that of TGF-beta(1) may be related to the therapeutic effect for psoriasis. The finding that IL-10 induction was more marked after IPL treatment than after PDT suggests that other mechanisms than IL-10 induction in keratinocytes after PDT may participate in the anti-inflammatory effect of PDT.
根据光动力治疗（PDT）中光剂量和光敏剂浓度的不同，在接受PDT治疗的细胞中可观察到大量与剂量相关的事件。亚致死剂量可能导致细胞因子释放改变，进而改变免疫反应，而非引起细胞死亡。
本研究旨在探讨在亚致死剂量下，强脉冲光（IPL）治疗或利用5-氨基酮戊酸（ALA）和IPL进行PDT后，培养的HaCaT细胞中细胞因子的表达情况。
将培养的HaCaT细胞分别用单纯IPL（4、8和12 J/cm²）或ALA-IPL PDT（100 μmol/L的ALA；0、4、8和12 J/cm²的IPL）进行处理。通过逆转录-聚合酶链反应和酶联免疫吸附测定法研究白细胞介素-10（IL-10）、转化生长因子-β1（TGF-β1）和肿瘤坏死因子-α（TNF-α）的表达。
IPL治疗后IL-10蛋白增加至5.95倍，PDT后增加至2.85倍。IPL治疗和PDT后，TGF-β1 mRNA和蛋白均有轻微增加，其中后者诱导的增加幅度稍大。PDT后TNF-α mRNA和蛋白无诱导或减少。
PDT后观察到IL-10和TGF-β1表达增加。IL-10的诱导可能有助于抗炎作用，这解释了PDT对炎症性皮肤病的治疗益处，而TGF-β1的诱导可能与对银屑病的治疗效果有关。IPL治疗后IL-10诱导比PDT后更明显这一发现表明，PDT后角质形成细胞中除IL-10诱导外的其他机制可能参与了PDT的抗炎作用。
