Momtaz Saeideh, Lall Namrita, Hussein Ahmed, Ostad Seyed Nasser, Abdollahi Mohammad
Department of Plant Science, Faculty of Natural and Agricultural Science, University of Pretoria, Pretoria, South Africa.
Pharmacogn Mag. 2010 Jan;6(21):34-41. doi: 10.4103/0973-1296.59964. Epub 2010 Feb 13.
The present study was undertaken to explore the possible biochemical activities of Hyaenanche globosa Lamb. and its compounds. Two different extracts (ethanol and dichloromethane) of four different parts (leaves, root, stem, and fruits) of H. globosa were evaluated for their possible antibacterial, antityrosinase, and anticancer (cytotoxicity) properties. Two pure compounds were isolated using column chromatographic techniques. Active extracts and pure compounds were investigated for their antioxidant effect on cultured 'Hela cells'. Antioxidant/oxidative properties of the ethanolic extract of the fruits of H. globosa and purified compounds were investigated using reactive oxygen species (ROS), ferric-reducing antioxidant power (FRAP), and lipid peroxidation thiobarbituric acid reactive substance (TBARS) assays. The ethanolic extract of the leaves and fruits of H. globosa showed the best activity, exhibiting a minimum inhibitory concentration (MIC) of 3.1 mg/ ml and a minimum bactericidal concentration (MBC) of 1.56 and 6.2 mg/ml, respectively, against M. smegmatis. The ethanolic extract of the fruits of H. globosa (F.E) showed the highest percentage of inhibitory activity of monophenolase (90.4% at 200 mug/ml). In addition, F.E exhibited 50% inhibitory concentration (IC(50)) of 37.7 mug/ml on the viability of 'HeLa cells' using cytotoxicity MTT assay. Subsequently, F.E was fractionated using phase-partitioning with n-hexane, ethyl acetate, and n-butanol. The cytotoxicity of these fractions were determined in vitro using different cancer cell lines. The n-hexane fraction exhibited the highest activity of toxicity. Therefore, this fraction was subjected to further separation by chromatographic methods. Two pure compounds known as: 'Tutin' and 'hyenanchin' were isolated and their structures were determined by NMR spectroscopic methods. Unpredictably, none of them showed significant (P < 0.01) inhibition on cell viability/proliferation at the concentrations that were used. F.E showed significant anti-tyrosinase, antibacterial, and cytotoxicity effects, therefore it can be considered as an effective inhibitor alone or in combination with other plant extracts.
本研究旨在探索球果寄生（Hyaenanche globosa Lamb.）及其化合物可能的生化活性。对球果寄生四个不同部位（叶、根、茎和果实）的两种不同提取物（乙醇提取物和二氯甲烷提取物）的抗菌、抗酪氨酸酶和抗癌（细胞毒性）特性进行了评估。使用柱色谱技术分离出两种纯化合物。研究了活性提取物和纯化合物对培养的“HeLa细胞”的抗氧化作用。采用活性氧（ROS）、铁还原抗氧化能力（FRAP）和脂质过氧化硫代巴比妥酸反应物质（TBARS）测定法，研究了球果寄生果实乙醇提取物和纯化化合物的抗氧化/氧化特性。球果寄生叶和果实的乙醇提取物表现出最佳活性，对耻垢分枝杆菌的最低抑菌浓度（MIC）分别为3.1 mg/ml，最低杀菌浓度（MBC）分别为1.56和6.2 mg/ml。球果寄生果实乙醇提取物（F.E）对单酚酶的抑制活性百分比最高（200 μg/ml时为90.4%）。此外，使用细胞毒性MTT测定法，F.E对“HeLa细胞”活力的50%抑制浓度（IC50）为37.7 μg/ml。随后，F.E用正己烷、乙酸乙酯和正丁醇进行相分离分级。使用不同癌细胞系在体外测定这些级分的细胞毒性。正己烷级分表现出最高的毒性活性。因此，该级分通过色谱方法进行进一步分离。分离出两种纯化合物，分别为“吐丁”和“球果寄生素”，并通过核磁共振光谱法确定了它们的结构。出乎意料的是，在所使用的浓度下，它们均未对细胞活力/增殖表现出显著（P < 0.01）抑制作用。F.E表现出显著的抗酪氨酸酶、抗菌和细胞毒性作用，因此它可单独或与其他植物提取物联合被视为一种有效的抑制剂。
