忍冬甲醇提取物对谷氨酸损伤的原代大鼠皮层细胞的神经保护活性。
Neuroprotective activity of the methanolic extract of Lonicera japonica in glutamate-injured primary rat cortical cells.
作者信息
Weon Jin Bae, Yang Hye Jin, Lee Bohyoung, Yun Bo-Ra, Ahn Ju Hee, Lee Hyeon Yong, Ma Choong Je
机构信息
Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Republic of Korea.
出版信息
Pharmacogn Mag. 2011 Oct;7(28):284-8. doi: 10.4103/0973-1296.90404.
BACKGROUND
We previously reported that the extracts of several Korean medicinal plants showed neuroprotective activity in glutamate-injured primary culutres of rat cortical cells.
OBJECTIVE
Among them, the effect of the methanolic extract of Lonicera japonica flower on the glutamate-induced neuronal cell death and its potential mechanism of action was investigated.
RESULTS
Treatment by the methanolic extract of L. japonica flower significantly protected neuronal cells against glutamate-induced excitotoxicity. It decreased the calcium influx that accompanies the glutamate induced excitotoxicity of neuronal cells, and inhibited the subsequent overproduction of nitric oxide, reactive oxygen species and peroxide to the level of control cells. In addition, it preserved cellular activity of superoxide dismutase, an antioxidative enzyme reduced by glutamate insult.
CONCLUSIONS
According to this data, the methanolic extract of L. japonica flower significantly protected neuronal cells against glutamate excitotoxicity via antioxidative activity.
背景
我们之前报道过，几种韩国药用植物的提取物在谷氨酸损伤的大鼠皮层细胞原代培养物中显示出神经保护活性。
目的
其中，研究了忍冬花花甲醇提取物对谷氨酸诱导的神经元细胞死亡的影响及其潜在作用机制。
结果
忍冬花花甲醇提取物处理显著保护神经元细胞免受谷氨酸诱导的兴奋毒性。它减少了伴随谷氨酸诱导的神经元细胞兴奋毒性的钙内流，并将随后一氧化氮、活性氧和过氧化物的过量产生抑制到对照细胞的水平。此外，它保留了超氧化物歧化酶的细胞活性，超氧化物歧化酶是一种因谷氨酸损伤而减少的抗氧化酶。
结论
根据这些数据，忍冬花花甲醇提取物通过抗氧化活性显著保护神经元细胞免受谷氨酸兴奋毒性。
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