Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA.
Biomed Microdevices. 2010 Oct;12(5):865-74. doi: 10.1007/s10544-010-9441-2.
Islet size has recently been demonstrated to be an important factor in determining human islet transplantation outcomes. In this study, a multi-layered microfluidic device was developed and quantified for size-based separation of a heterogeneous population of mouse islets. The device was fabricated using standard soft lithography and polydimethylsiloxane (PDMS). Size-based separation was first demonstrated via injection of a heterogeneous population of glass beads between 50-300 microm in diameter which were separated into five sub-populations based on their diameter. Next, a heterogeneous population of mouse pancreatic islets, between 50-250 microm in diameter was separated into four sub-populations. Throughout this process the islets remained intact without any signs of damage, as indicated by cell viability staining. Islet glucose-stimulated insulin secretion of each sub-population of islets was also evaluated demonstrating that islets smaller than 150 microm have superior stimulation indexes (SI) compared to islets larger than 150 microm. In this study, we found that islets between 100 microm and 150 microm in diameter had the greatest SI value in a heterogeneous population of islets.
胰岛大小最近被证明是决定人类胰岛移植结果的一个重要因素。在这项研究中，开发并量化了一种多层微流控装置，用于基于大小的分离异质群体的小鼠胰岛。该设备使用标准的软光刻和聚二甲基硅氧烷（PDMS）制造。通过在直径为 50-300 微米的范围内注射异质群体的玻璃珠，首先证明了基于大小的分离，这些玻璃珠被分离成五个亚群，基于它们的直径。接下来，将直径在 50-250 微米之间的异质群体的小鼠胰岛分离成四个亚群。在整个过程中，胰岛保持完整，没有任何损伤的迹象，如细胞活力染色所示。还评估了每个胰岛亚群的胰岛葡萄糖刺激胰岛素分泌，结果表明，直径小于 150 微米的胰岛与直径大于 150 微米的胰岛相比具有更高的刺激指数（SI）。在这项研究中，我们发现，在异质群体的胰岛中，直径在 100 微米到 150 微米之间的胰岛具有最大的 SI 值。
