Morphology and Evolution of Animals, Philipps-Universität Marburg, Karl-von-Frisch-Strasse 8, Marburg, Germany.
Dev Dyn. 2010 Jul;239(7):1967-76. doi: 10.1002/dvdy.22331.
Molecular data for nephridial development in polychaetes are not available yet. The scope of our work was to establish a reference system for future investigations using two markers for nephridial development: beta-tubulin as marker for cilia and alkaline phosphatase (AP) activity for secretory epithelia. The markers identified, unexpectedly, three consecutively forming generations of nephridia: (1) a transitory unciliated, but AP-positive head kidney, (2) a transitory larval nephridium, which undergoes a morphological transition from a protonephridium to a funnelled nephridium concomitant with the development of the coelomic cavity and finally, (3) the serially arranged metanephridia. The spatial arrangement of larval and definitive nephridia, revealed an up to now unknown developmental boundary between the synchronously forming larval and the serially proliferating definitive segments. Development of three consecutive sets of nephridia with different morphology and biochemical properties was unexpected and reveals an interesting multistep process in the development of excretory structures in Platynereis.
目前还没有关于多毛类动物肾管发育的分子数据。我们的工作范围是建立一个参考系统，以便未来使用两种用于肾管发育的标记物进行研究：β-微管蛋白作为纤毛的标记物和碱性磷酸酶（AP）活性用于分泌上皮。令人意外的是，我们用这两种标记物鉴定出了连续形成的三代肾管：（1）一个短暂的无纤毛但 AP 阳性的头肾，（2）一个短暂的幼虫肾管，它经历了从原肾到漏斗肾的形态转变，伴随着体腔的发育，最后，（3）串联排列的后肾。幼虫和终末肾管的空间排列揭示了在同步形成的幼虫和串联增殖的终末段之间存在一个迄今为止未知的发育边界。具有不同形态和生化特性的三组连续肾管的发育是出乎意料的，这揭示了扁形动物排泄结构发育中的一个有趣的多步骤过程。
