Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, Oklahoma 73019, USA.
Biochemistry. 2010 Jul 27;49(29):5989-97. doi: 10.1021/bi100393r.
The Bacillus subtilis phage phi29 packaging motor requires prohead RNA for genome encapsidation. The nuclear magnetic resonance structure of the prohead RNA E-loop hairpin, r(5'AUUGAGUU), is presented and compared to predictions from MC-SYM. The prohead RNA E-loop hairpins contain sequences similar to rRNA hairpins. Comparison of predicted and experimentally determined prohead and ribosomal hairpin structures reveals that sequence similarity is a stronger determinant of hairpin structural similarity than grouping similar types of RNA. All the hairpins contain a U-turn motif but differ in the first noncanonical pair and backbone orientation. These structures provide benchmarks for further improvements in RNA structure predictions from sequence.
枯草芽孢杆菌噬菌体 phi29 包装马达需要头部 RNA 进行基因组包装。本文呈现了头部 RNA E 环发夹 r(5'AUUGAGUU)的核磁共振结构，并与 MC-SYM 的预测结果进行了比较。头部 RNA E 环发夹包含与 rRNA 发夹相似的序列。预测的和实验确定的头部和核糖体发夹结构的比较表明，序列相似性是发夹结构相似性的更强决定因素，而不是将相似类型的 RNA 分组。所有的发夹都包含 U 形转弯模体，但第一个非canonical 对和骨架方向不同。这些结构为进一步提高基于序列的 RNA 结构预测提供了基准。
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