Laboratory of Food Microbiology, Wageningen University,Bomenweg 2, 6703HD Wageningen, The Netherlands.
J Agric Food Chem. 2010 Jul 14;58(13):7649-56. doi: 10.1021/jf101379y.
Tempe extracts can inhibit the adhesion of enterotoxigenic Escherichia coli (ETEC) to intestinal cells and thereby can play a role in controlling ETEC-induced diarrhea. The component responsible for this adhesion inhibition activity is still unknown. This research describes the purification and partial characterization of this bioactive component of tempe. After heating, defatting, and protease treatment, the extracts were found to remain active. However, after treatment with polysaccharide-degrading enzyme mixtures the bioactivity was lost. Ultrafiltration revealed the active component to be >30 kDa. Further purification of the bioactive tempe extracts yielded an active fraction with an increased carbohydrate content of higher arabinose content than the nonactive fractions. In conclusion, the bioactive component contains arabinose and originates from the arabinan or arabinogalactan side chain of the pectic cell wall polysaccharides of the soybeans, which is probably released or formed during fermentation by enzymatic modifications.
豆豉提取物可抑制肠产毒性大肠杆菌（ETEC）黏附肠道细胞，从而在控制 ETEC 诱导的腹泻方面发挥作用。但负责这种黏附抑制活性的成分尚不清楚。本研究描述了豆豉这种生物活性成分的纯化和部分特性。经加热、脱脂和蛋白酶处理后，提取物仍保持活性。然而，用多糖降解酶混合物处理后，生物活性丧失。超滤显示活性成分大于 30 kDa。进一步纯化豆豉提取物得到了具有活性的级分，其碳水化合物含量增加，阿拉伯糖含量高于非活性级分。总之，生物活性成分含有阿拉伯糖，来源于大豆果胶细胞壁多糖的阿拉伯聚糖或阿拉伯半乳聚糖侧链，可能在发酵过程中通过酶修饰释放或形成。
