Department of Medicine, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark, New Jersey, USA.
PLoS One. 2010 Jun 11;5(6):e10980. doi: 10.1371/journal.pone.0010980.
Knowledge of the entire protein content, the proteome, of normal human cerebrospinal fluid (CSF) would enable insights into neurologic and psychiatric disorders. Until now technologic hurdles and access to true normal samples hindered attaining this goal.
We applied immunoaffinity separation and high sensitivity and resolution liquid chromatography-mass spectrometry to examine CSF from healthy normal individuals. 2630 proteins in CSF from normal subjects were identified, of which 56% were CSF-specific, not found in the much larger set of 3654 proteins we have identified in plasma. We also examined CSF from groups of subjects previously examined by others as surrogates for normals where neurologic symptoms warranted a lumbar puncture but where clinical laboratory were reported as normal. We found statistically significant differences between their CSF proteins and our non-neurological normals. We also examined CSF from 10 volunteer subjects who had lumbar punctures at least 4 weeks apart and found that there was little variability in CSF proteins in an individual as compared to subject to subject.
Our results represent the most comprehensive characterization of true normal CSF to date. This normal CSF proteome establishes a comparative standard and basis for investigations into a variety of diseases with neurological and psychiatric features.
了解正常人类脑脊液（CSF）的全部蛋白质含量，即蛋白质组，将有助于深入了解神经和精神疾病。直到现在，技术障碍和获得真正正常样本的途径阻碍了实现这一目标。
我们应用免疫亲和分离和高灵敏度、高分辨率的液相色谱-质谱法来检测健康正常个体的 CSF。从正常受试者的 CSF 中鉴定出 2630 种蛋白质，其中 56%是 CSF 特异性的，在我们在血浆中鉴定出的 3654 种蛋白质中没有发现。我们还检查了先前由其他人作为正常替代者检查的受试者的 CSF，这些受试者的神经症状需要进行腰椎穿刺，但临床实验室报告正常。我们发现他们的 CSF 蛋白与我们的非神经正常受试者之间存在统计学上的显著差异。我们还检查了 10 名志愿者的 CSF，他们至少相隔 4 周进行了腰椎穿刺，发现与个体间相比，个体 CSF 蛋白的变化很小。
我们的结果代表了迄今为止对真正正常 CSF 的最全面描述。这个正常 CSF 蛋白质组建立了一个比较标准和基础，用于研究具有神经和精神特征的各种疾病。
